Correlations between internal mobility and stability of globular proteins.

K. Wüthrich, G. Wagner, R. Richarz, Werner Braun

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The recent work is surveyed which leads to the suggestions that the conformation of globular proteins in solution corresponds to a dynamic ensemble of rapidly interconverting spatial structures, that clusters of hydrophobic amino acid side chains have an important role in the architecture of protein molecules, and that mechanistic aspects of protein denaturation can be correlated with internal mobility seen in the native conformation. These conclusions resulted originally from high resolution 1H nuclear magnetic resonance (NMR) studies of aromatic ring mobility, exchange of interior amide protons and thermal denaturation of the basic pancreatic trypsin inhibitor and a group of related proteins. Various new approaches to further characterize proteins in solution have now been taken and preliminary data are presented. These include computer graphics to outline hydrophobic clusters in globular protein structures, high resolution 1H-NMR experiments at variable hydrostatic pressure and 13C-NMR relaxation measurements. At the present early stage of these new investigations it appears that the hydrophobic cluster model for globular proteins is compatible with the data obtained.

Original languageEnglish (US)
Pages (from-to)549-560
Number of pages12
JournalBiophysical Journal
Volume32
Issue number1
StatePublished - Oct 1980
Externally publishedYes

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Protein Stability
Magnetic Resonance Spectroscopy
Proteins
Computer Graphics
Protein Denaturation
Protein Conformation
Aprotinin
Hydrostatic Pressure
Amides
Protons
Hot Temperature
Amino Acids

ASJC Scopus subject areas

  • Biophysics

Cite this

Correlations between internal mobility and stability of globular proteins. / Wüthrich, K.; Wagner, G.; Richarz, R.; Braun, Werner.

In: Biophysical Journal, Vol. 32, No. 1, 10.1980, p. 549-560.

Research output: Contribution to journalArticle

Wüthrich, K, Wagner, G, Richarz, R & Braun, W 1980, 'Correlations between internal mobility and stability of globular proteins.', Biophysical Journal, vol. 32, no. 1, pp. 549-560.
Wüthrich, K. ; Wagner, G. ; Richarz, R. ; Braun, Werner. / Correlations between internal mobility and stability of globular proteins. In: Biophysical Journal. 1980 ; Vol. 32, No. 1. pp. 549-560.
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