Correlative aspects of luteinizing hormone releasing hormone sensitivity and cytoplasmic estrogen receptor concentration in the anterior pituitary and hypothalamus of the cycling rat

G. H. Greeley, T. G. Muldoon, V. B. Mahesh

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    24 Citations (Scopus)

    Abstract

    Pituitary responsiveness to an intra arterial injection of synthetic LH RH (50 ng) was examined at critical times during the rat 4 day estrous cycle. LH release was maximal and prolonged on proestrus; the response on the other days was of short duration and the order of magnitude was estrus > diestrus 1 > diestrus 2. Sera FSH levels did not rise significantly until 60 min after an inter arterial injection of LH RH. Pituitary and hypothalamic cytoplasmic receptor levels were measured at identical times throughout the estrous cycle using protamine sulfate precipitation and Scatchard plot analysis. Hypothalamic and pituitary estrophile levels showed significant decreases which started on the afternoon of diestrus 2 and continued until mid proestrus. Receptor depletion was followed by a replenishment process which began on late proestrus and continued through mid diestrus 2 at both tissue sites. This pattern correlates well with the changing plasma estradiol level in accordance with the current concept of receptor depletion by nuclear translocation followed by cytoplasmic receptor resynthesis. The authors conclude that the change in pituitary receptor population correlates with the tissue's sensitivity to exogenous LH RH, lending further support to the concept of a direct functional effect of estradiol at the pituitary level.

    Original languageEnglish (US)
    Pages (from-to)505-512
    Number of pages8
    JournalBiology of Reproduction
    Volume13
    Issue number5
    StatePublished - 1975

    Fingerprint

    Anterior Hypothalamus
    Diestrus
    Cytoplasmic and Nuclear Receptors
    Gonadotropin-Releasing Hormone
    Proestrus
    Estrogen Receptors
    Estrous Cycle
    Estradiol
    Intra-Arterial Injections
    Protamines
    Estrus
    Injections
    Serum
    Population

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Correlative aspects of luteinizing hormone releasing hormone sensitivity and cytoplasmic estrogen receptor concentration in the anterior pituitary and hypothalamus of the cycling rat. / Greeley, G. H.; Muldoon, T. G.; Mahesh, V. B.

    In: Biology of Reproduction, Vol. 13, No. 5, 1975, p. 505-512.

    Research output: Contribution to journalArticle

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