Correlative gene expression to protective seroconversion in rift valley fever vaccinates

Richard C. Laughlin, Kenneth L. Drake, John C. Morrill, L. Garry Adams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rift Valley fever Virus (RVFV), a negative-stranded RNA virus, is the etiological agent of the vector-borne zoonotic disease, Rift Valley fever (RVF). In both humans and livestock, protective immunity can be achieved through vaccination. Earlier and more recent vaccine trials in cattle and sheep demonstrated a strong neutralizing antibody and total IgG response induced by the RVF vaccine, authentic recombinant MP-12 (arMP-12). From previous work, protective immunity in sheep and cattle vaccinates normally occurs from 7 to 21 days after inoculation with arMP-12. While the serology and protective response induced by arMP-12 has been studied, little attention has been paid to the underlying molecular and genetic events occurring prior to the serologic immune response. To address this, we isolated RNA from whole blood of vaccinated calves over a time course of 21 days before and after vaccination with arMP-12. The time course RNAs were sequenced by RNASeq and bioinformatically analyzed. Our results revealed time-dependent activation or repression of numerous gene ontologies and pathways related to the vaccine induced immune response and its regulation. Additional bioinformatic analyses identified a correlative relationship between specific host immune response genes and protective immunity prior to the detection of protective serum neutralizing antibody responses. These results contribute an important proof of concept for identifying molecular and genetic components underlying the immune response to RVF vaccination and protection prior to serologic detection.

Original languageEnglish (US)
Article numbere0147027
JournalPLoS One
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2016

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Rift Valley Fever
Rift Valley fever
seroconversion
Gene expression
Immunity
Vaccination
vaccination
immune response
RNA
Neutralizing Antibodies
Viruses
Gene Expression
gene expression
immunity
Molecular Biology
Sheep
Vaccines
Genes
Rift Valley fever virus
neutralizing antibodies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Correlative gene expression to protective seroconversion in rift valley fever vaccinates. / Laughlin, Richard C.; Drake, Kenneth L.; Morrill, John C.; Adams, L. Garry.

In: PLoS One, Vol. 11, No. 1, e0147027, 01.01.2016.

Research output: Contribution to journalArticle

Laughlin, Richard C. ; Drake, Kenneth L. ; Morrill, John C. ; Adams, L. Garry. / Correlative gene expression to protective seroconversion in rift valley fever vaccinates. In: PLoS One. 2016 ; Vol. 11, No. 1.
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