Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues

Research output: Contribution to journalArticlepeer-review


Correlative light-electron microscopy (CLEM) has evolved in the last decades, especially after significant developments in sample preparation, imaging acquisition, software, spatial resolution, and equipment, including confocal, live-cell, super-resolution, and electron microscopy (scanning, transmission, focused ion beam, and cryo-electron microscopy). However, the recent evolution of different laser-related techniques, such as mass spectrometry imaging (MSI) and laser capture microdissection, could further expand spatial imaging capabilities into high-resolution OMIC approaches such as proteomic, lipidomics, small molecule, and drug discovery. Here, we will describe a protocol to integrate the detection of rare viral reservoirs with imaging mass spectrometry.

Original languageEnglish (US)
Pages (from-to)93-110
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2024


  • AIDS
  • Colocalization
  • Cure
  • Electron microscopy
  • Eradication
  • HIV-1
  • Mass spectrometry imaging
  • Confocal

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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