TY - JOUR
T1 - Corrigendum to “Early life neuroimmune challenge protects the brain after sepsis in adult rats” [Neurochem. Int. 2020 May 135 104712] (Neurochemistry International (2020) 135, (S0197018620301030), (10.1016/j.neuint.2020.104712))
AU - Florentino, Drielly
AU - Della Giustina, Amanda
AU - de Souza Goldim, Mariana Pereira
AU - Danielski, Lucineia Gainski
AU - de Oliveira Junior, Aloir Neri
AU - Joaquim, Larissa
AU - Bonfante, Sandra
AU - Biehl, Erica
AU - da Rosa, Naiana
AU - Fernandes, Deisy
AU - Gava, Fernanda Frederico
AU - Michels, Monique
AU - Fortunato, Jucelia Jeremias
AU - Réus, Gislaine Zilli
AU - Valvassori, Samira Silva
AU - Quevedo, Joao
AU - Dal-Pizzol, Felipe
AU - Barichello, Tatiana
AU - Petronilho, Fabricia
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/7
Y1 - 2020/7
N2 - 1. The name of a co-author (Samira S Valvassori) was written wrong. In the article, the name is correct, but in the search system, the name is wrong. 2. The figures do not follow the sequence shown in the legend. Bellow the correct sequence of the figure and respective legends: [Figure presented] Fig. 1. This figure represents a timeline of the two sets of experiments. The figure 1A (experiment 1) shows a LPS-preconditioning or saline administration in rats with 14 days old. On the 60 days old, the animals were submitted to the CLP or sham procedure and 24 hours after, CSF, hippocampus and prefrontal cortex were isolated to evaluation of neuroinflammation, oxidative stress, BBB permeability and epigenetic alterations. In parallel to this, after CLP or sham the rats were accompanied during 10 days to survival analyses and with 70 days old, were submitted to behavioral tests and BDNF level was verified in hippocampus and prefrontal cortex. The figure 2 (experiment 2) shows the LPS-preconditioning or saline administration in rats with 57 days old. On the 60 days old, the animals were submitted to the CLP or sham procedure and 24 hours after, CSF, hippocampus and prefrontal cortex were isolated to evaluation of cytokines, oxidative damage and nitrite/nitrate concentration. [Figure presented] Fig. 2. Cytokines levels of TNF-α (A and B), IL-1β (C and D) and IL-6 (E and F) in the CSF samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A, C and E) and 57 days old (B, D and F). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 3. Nitrite/nitrate concentration in the CSF samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A) and 57 days old (B). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 4. Lipid peroxidation (A and B), protein carbonylation (C and D) and sulfhydryl grupaments integrity (E and F) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A, C and E) and 57 days old (B, D and F). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 5. Superoxide dismutase (A) and catalase activity (B) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 6. Blood brain barrier permeability (A) and infiltration of neutrophils verification by myeloperoxidase activity (B) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 7. HDAC (A), HAT (B) and DNMT activity (C) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 8. BDNF levels in the hippocampus and prefrontal cortex samples 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 9. Number of crossings (A) and rearings (B) in the habituation session of the open field test and short-term (C) and long-term (D) object recognition index 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is presented as mean ± standard deviation, analyzed by Mann Whitney and Wilcoxon test. *p < 0.05 compared to training session. [Figure presented] Fig. 10. Kaplan-Meier curve for survival analysis during 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data analyzed by long-rank. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 11. This figure represents a summary of protection exerted by LPS preconditioning in early life against CLP impairments in adulthood. Rats subjected to an LPS challenge in early life, presented significant neuroprotection at 24 hours after polymicrobial sepsis in adulthood rats. In this scenario, occurs attenuation of neuroinflammation, oxidative damage, BBB permeability, epigenetic enzymes and an increase of antioxidant enzymes. These acute response, results in protection on long term alterations in rats in 10 days after CLP with increase of BDNF levels, memory and survival. BBB, blood brain barrier; BDNF, brain-derived neurotrophic factor; CAT, catalase; CLP, cecal ligation and perforation, CSF, cerebrospinal fluid; DNMT, DNA methyltransferase; HDAC, histone desacetylase; Hip, hippocampus; IL-6, interleukin-6; LPS, lipopolysaccharide; OFT, open field test; ORT, object recognition test; PFC, prefrontal cortex; STM, short term memory; TNF-α, tumor necrosis factor alpha.
AB - 1. The name of a co-author (Samira S Valvassori) was written wrong. In the article, the name is correct, but in the search system, the name is wrong. 2. The figures do not follow the sequence shown in the legend. Bellow the correct sequence of the figure and respective legends: [Figure presented] Fig. 1. This figure represents a timeline of the two sets of experiments. The figure 1A (experiment 1) shows a LPS-preconditioning or saline administration in rats with 14 days old. On the 60 days old, the animals were submitted to the CLP or sham procedure and 24 hours after, CSF, hippocampus and prefrontal cortex were isolated to evaluation of neuroinflammation, oxidative stress, BBB permeability and epigenetic alterations. In parallel to this, after CLP or sham the rats were accompanied during 10 days to survival analyses and with 70 days old, were submitted to behavioral tests and BDNF level was verified in hippocampus and prefrontal cortex. The figure 2 (experiment 2) shows the LPS-preconditioning or saline administration in rats with 57 days old. On the 60 days old, the animals were submitted to the CLP or sham procedure and 24 hours after, CSF, hippocampus and prefrontal cortex were isolated to evaluation of cytokines, oxidative damage and nitrite/nitrate concentration. [Figure presented] Fig. 2. Cytokines levels of TNF-α (A and B), IL-1β (C and D) and IL-6 (E and F) in the CSF samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A, C and E) and 57 days old (B, D and F). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 3. Nitrite/nitrate concentration in the CSF samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A) and 57 days old (B). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 4. Lipid peroxidation (A and B), protein carbonylation (C and D) and sulfhydryl grupaments integrity (E and F) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old (A, C and E) and 57 days old (B, D and F). Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 5. Superoxide dismutase (A) and catalase activity (B) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 6. Blood brain barrier permeability (A) and infiltration of neutrophils verification by myeloperoxidase activity (B) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 7. HDAC (A), HAT (B) and DNMT activity (C) in the hippocampus and prefrontal cortex samples 24 hours after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 8. BDNF levels in the hippocampus and prefrontal cortex samples 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is expressed as mean ± standard deviation, analyzed by one-way ANOVA with Tukey post-hoc test. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 9. Number of crossings (A) and rearings (B) in the habituation session of the open field test and short-term (C) and long-term (D) object recognition index 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data is presented as mean ± standard deviation, analyzed by Mann Whitney and Wilcoxon test. *p < 0.05 compared to training session. [Figure presented] Fig. 10. Kaplan-Meier curve for survival analysis during 10 days after CLP or sham procedure in rats with 60 days old preconditioned with LPS or saline on the 14 days old. Data analyzed by long-rank. *p < 0.05 compared to saline + sham group, & p < 0.05 compared to LPS + sham group and #p < 0.05 compared to saline + CLP. [Figure presented] Fig. 11. This figure represents a summary of protection exerted by LPS preconditioning in early life against CLP impairments in adulthood. Rats subjected to an LPS challenge in early life, presented significant neuroprotection at 24 hours after polymicrobial sepsis in adulthood rats. In this scenario, occurs attenuation of neuroinflammation, oxidative damage, BBB permeability, epigenetic enzymes and an increase of antioxidant enzymes. These acute response, results in protection on long term alterations in rats in 10 days after CLP with increase of BDNF levels, memory and survival. BBB, blood brain barrier; BDNF, brain-derived neurotrophic factor; CAT, catalase; CLP, cecal ligation and perforation, CSF, cerebrospinal fluid; DNMT, DNA methyltransferase; HDAC, histone desacetylase; Hip, hippocampus; IL-6, interleukin-6; LPS, lipopolysaccharide; OFT, open field test; ORT, object recognition test; PFC, prefrontal cortex; STM, short term memory; TNF-α, tumor necrosis factor alpha.
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U2 - 10.1016/j.neuint.2020.104732
DO - 10.1016/j.neuint.2020.104732
M3 - Comment/debate
C2 - 32276800
AN - SCOPUS:85082830621
SN - 0197-0186
VL - 137
JO - Neurochemistry International
JF - Neurochemistry International
M1 - 104732
ER -