Costunolide and Dehydrocostuslactone, two natural sesquiterpene lactones, ameliorate the inflammatory process associated to experimental pleurisy in mice

Elena Butturini, Rosanna Di Paola, Hisanori Suzuki, Irene Paterniti, Akbar Ahmad, Sofia Mariotto, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of costunolide (CS) and dehydrocostuslactone (DCE) a well-known sesquiterpene lactones contained in many plants, in a model of lung injury induced by carrageenan administration in the mice. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of polymorphonuclear cells (PMNs) as well as an infiltration of PMNs in lung tissues and increased production of tumour necrosis factor α (TNF-α). All parameters of inflammation were attenuated by CS and DCE (15 mg/kg 10% DMSO i.p.) administered 1 h before carrageenan. Carrageenan induced an up regulation of the intracellular adhesion molecules-1 (ICAM-1) and P-selectin, as well as nitrotyrosine and poly (ADP-ribose) (PAR) as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, P-selectin, nitrotyrosine and PAR was reduced by CS and DCE. Additionally we show that this inflammatory events were associated with NF-κB and STAT3 activation and these sesquiterpenes down-regulated it. Taken together, ours results clearly shown that CS and DCE may offer a novel therapeutic approach for the management of inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalEuropean Journal of Pharmacology
Volume730
Issue number1
DOIs
StatePublished - May 5 2014
Externally publishedYes

Fingerprint

Pleurisy
Sesquiterpenes
Carrageenan
Lactones
Pleural Cavity
P-Selectin
Poly Adenosine Diphosphate Ribose
Lung
Lung Injury
Disease Management
Dimethyl Sulfoxide
Up-Regulation
Tumor Necrosis Factor-alpha
Cell Count
Staining and Labeling
Inflammation
Injections
costunolide
dehydrocostus lactone
3-nitrotyrosine

Keywords

  • Costunolide
  • Dehydrocostuslactone
  • Inflammation
  • NF-κB
  • Peroxynitrite
  • STAT3

ASJC Scopus subject areas

  • Pharmacology
  • Medicine(all)

Cite this

Costunolide and Dehydrocostuslactone, two natural sesquiterpene lactones, ameliorate the inflammatory process associated to experimental pleurisy in mice. / Butturini, Elena; Di Paola, Rosanna; Suzuki, Hisanori; Paterniti, Irene; Ahmad, Akbar; Mariotto, Sofia; Cuzzocrea, Salvatore.

In: European Journal of Pharmacology, Vol. 730, No. 1, 05.05.2014, p. 107-115.

Research output: Contribution to journalArticle

Butturini, Elena ; Di Paola, Rosanna ; Suzuki, Hisanori ; Paterniti, Irene ; Ahmad, Akbar ; Mariotto, Sofia ; Cuzzocrea, Salvatore. / Costunolide and Dehydrocostuslactone, two natural sesquiterpene lactones, ameliorate the inflammatory process associated to experimental pleurisy in mice. In: European Journal of Pharmacology. 2014 ; Vol. 730, No. 1. pp. 107-115.
@article{e448db7dce5c4d398948339fbc21c461,
title = "Costunolide and Dehydrocostuslactone, two natural sesquiterpene lactones, ameliorate the inflammatory process associated to experimental pleurisy in mice",
abstract = "The aim of this study was to investigate the effect of costunolide (CS) and dehydrocostuslactone (DCE) a well-known sesquiterpene lactones contained in many plants, in a model of lung injury induced by carrageenan administration in the mice. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of polymorphonuclear cells (PMNs) as well as an infiltration of PMNs in lung tissues and increased production of tumour necrosis factor α (TNF-α). All parameters of inflammation were attenuated by CS and DCE (15 mg/kg 10{\%} DMSO i.p.) administered 1 h before carrageenan. Carrageenan induced an up regulation of the intracellular adhesion molecules-1 (ICAM-1) and P-selectin, as well as nitrotyrosine and poly (ADP-ribose) (PAR) as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, P-selectin, nitrotyrosine and PAR was reduced by CS and DCE. Additionally we show that this inflammatory events were associated with NF-κB and STAT3 activation and these sesquiterpenes down-regulated it. Taken together, ours results clearly shown that CS and DCE may offer a novel therapeutic approach for the management of inflammatory diseases.",
keywords = "Costunolide, Dehydrocostuslactone, Inflammation, NF-κB, Peroxynitrite, STAT3",
author = "Elena Butturini and {Di Paola}, Rosanna and Hisanori Suzuki and Irene Paterniti and Akbar Ahmad and Sofia Mariotto and Salvatore Cuzzocrea",
year = "2014",
month = "5",
day = "5",
doi = "10.1016/j.ejphar.2014.02.031",
language = "English (US)",
volume = "730",
pages = "107--115",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Costunolide and Dehydrocostuslactone, two natural sesquiterpene lactones, ameliorate the inflammatory process associated to experimental pleurisy in mice

AU - Butturini, Elena

AU - Di Paola, Rosanna

AU - Suzuki, Hisanori

AU - Paterniti, Irene

AU - Ahmad, Akbar

AU - Mariotto, Sofia

AU - Cuzzocrea, Salvatore

PY - 2014/5/5

Y1 - 2014/5/5

N2 - The aim of this study was to investigate the effect of costunolide (CS) and dehydrocostuslactone (DCE) a well-known sesquiterpene lactones contained in many plants, in a model of lung injury induced by carrageenan administration in the mice. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of polymorphonuclear cells (PMNs) as well as an infiltration of PMNs in lung tissues and increased production of tumour necrosis factor α (TNF-α). All parameters of inflammation were attenuated by CS and DCE (15 mg/kg 10% DMSO i.p.) administered 1 h before carrageenan. Carrageenan induced an up regulation of the intracellular adhesion molecules-1 (ICAM-1) and P-selectin, as well as nitrotyrosine and poly (ADP-ribose) (PAR) as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, P-selectin, nitrotyrosine and PAR was reduced by CS and DCE. Additionally we show that this inflammatory events were associated with NF-κB and STAT3 activation and these sesquiterpenes down-regulated it. Taken together, ours results clearly shown that CS and DCE may offer a novel therapeutic approach for the management of inflammatory diseases.

AB - The aim of this study was to investigate the effect of costunolide (CS) and dehydrocostuslactone (DCE) a well-known sesquiterpene lactones contained in many plants, in a model of lung injury induced by carrageenan administration in the mice. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of polymorphonuclear cells (PMNs) as well as an infiltration of PMNs in lung tissues and increased production of tumour necrosis factor α (TNF-α). All parameters of inflammation were attenuated by CS and DCE (15 mg/kg 10% DMSO i.p.) administered 1 h before carrageenan. Carrageenan induced an up regulation of the intracellular adhesion molecules-1 (ICAM-1) and P-selectin, as well as nitrotyrosine and poly (ADP-ribose) (PAR) as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, P-selectin, nitrotyrosine and PAR was reduced by CS and DCE. Additionally we show that this inflammatory events were associated with NF-κB and STAT3 activation and these sesquiterpenes down-regulated it. Taken together, ours results clearly shown that CS and DCE may offer a novel therapeutic approach for the management of inflammatory diseases.

KW - Costunolide

KW - Dehydrocostuslactone

KW - Inflammation

KW - NF-κB

KW - Peroxynitrite

KW - STAT3

UR - http://www.scopus.com/inward/record.url?scp=84896552191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896552191&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2014.02.031

DO - 10.1016/j.ejphar.2014.02.031

M3 - Article

C2 - 24625594

AN - SCOPUS:84896552191

VL - 730

SP - 107

EP - 115

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -