Abstract
The covalent interaction of chloroacetic acid with rat liver lipids was studied in vivo. Rats were given a single oral dose (8.75 mg/kg, 50 μCi) of 1‐[14C]chloroacetic acid and sacrificed after 24 hours. Lipids extracted from the livers were separated into neutral lipids and phospholipids by solid‐phase extraction using sep‐pak silica cartridges. The neutral lipid fraction was further fractionated by preparative thin‐layer chromatography followed by reverse‐phase high‐performance liquid chromatography. The fraction corresponding to the retention time of standard cholesteryl chloroacetate gave a pseudomolecular ion peak at m/z 480/482 ratio: (3:1) on ammonia chemical ionization mass spectrometry, and the fragmentation pattern was found to be similar to that of the standard sample. Under similar conditions, acetic acid resulted in the formation of cholesteryl acetate. The effect of such conjugation reactions on the cell membrane and their contribution to toxicity is presently unknown.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 189-193 |
| Number of pages | 5 |
| Journal | Journal of biochemical toxicology |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1989 |
Keywords
- Acetic Acid
- Chloroacetic Acid
- Cholesterol Conjugates
- Cholesteryl Acetate
- Cholesteryl Chloroacetate
ASJC Scopus subject areas
- Toxicology