Covalent interaction of chloroacetic and acetic acids with cholesterol

Hari K. Bhat, G. A.S. Ansari

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The covalent interaction of chloroacetic acid with rat liver lipids was studied in vivo. Rats were given a single oral dose (8.75 mg/kg, 50 μCi) of 1‐[14C]chloroacetic acid and sacrificed after 24 hours. Lipids extracted from the livers were separated into neutral lipids and phospholipids by solid‐phase extraction using sep‐pak silica cartridges. The neutral lipid fraction was further fractionated by preparative thin‐layer chromatography followed by reverse‐phase high‐performance liquid chromatography. The fraction corresponding to the retention time of standard cholesteryl chloroacetate gave a pseudomolecular ion peak at m/z 480/482 ratio: (3:1) on ammonia chemical ionization mass spectrometry, and the fragmentation pattern was found to be similar to that of the standard sample. Under similar conditions, acetic acid resulted in the formation of cholesteryl acetate. The effect of such conjugation reactions on the cell membrane and their contribution to toxicity is presently unknown.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalJournal of biochemical toxicology
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1989

Keywords

  • Acetic Acid
  • Chloroacetic Acid
  • Cholesterol Conjugates
  • Cholesteryl Acetate
  • Cholesteryl Chloroacetate

ASJC Scopus subject areas

  • Toxicology

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