Covalent structure of the gamma chain of the A subunit of cholera toxin.

L. K. Duffy, Johnny Peterson, A. Kurosky

Research output: Contribution to journalArticle

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Abstract

The complete amino acid sequence of the gamma chain of the A subunit of cholera enterotoxin was determined: NH2-Met-Ser-Asn-Thr-Cys-Asp-Glu-Lys-Thr-Gln-Ser-Leu-Gly-Val-Lys-Phe-Leu-Asp-Glu -Tyr-Gln-Ser-Lys-Val-Lys-Arg-Gln-Ile-Phe-Ser-Gly-Tyr-Gln-Ser-Ile-Asp-Asp-Thr- His-Asn-Arg-Ile-Lys-Asn-Glu-Leu-COOH. This amino acid sequence was established in part by automated sequence analysis of intact gamma chain as well as of clostripain fragments and tryptic fragments generated from succinylated gamma chain. Smaller peptides from chymotryptic and Staphylococcus aureus protease hydrolyses provided the necessary overlaps. The gamma chain represents a segment of 46 amino acid residues (Mr = 5,395) from the carboxyl-terminal region of the precursor single-chain A subunit of cholera toxin (Duffy, L. K., Peterson, J. W., and Kurosky, A. (1981) FEBS Lett. 126, 187-190). Alignment of the gamma chain region of cholera toxin to a comparable chain region of 36 residues previously reported for heat-labile enterotoxin of Escherichia coli revealed an identity of 31%. The chemical similarity between the gamma chain regions of these two enterotoxins was thus considerably less than that of their beta chains which were shown to be 79% identical.

Original languageEnglish (US)
Pages (from-to)12252-12256
Number of pages5
JournalJournal of Biological Chemistry
Volume256
Issue number23
StatePublished - Dec 10 1981
Externally publishedYes

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Enterotoxins
Cholera Toxin
clostripain
Amino Acid Sequence
Amino Acids
Sequence Analysis
Staphylococcus aureus
Peptide Hydrolases
Hot Temperature
Escherichia coli
Peptides
Hydrolysis
serinamide
Vibrio cholerae stN protein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Covalent structure of the gamma chain of the A subunit of cholera toxin. / Duffy, L. K.; Peterson, Johnny; Kurosky, A.

In: Journal of Biological Chemistry, Vol. 256, No. 23, 10.12.1981, p. 12252-12256.

Research output: Contribution to journalArticle

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abstract = "The complete amino acid sequence of the gamma chain of the A subunit of cholera enterotoxin was determined: NH2-Met-Ser-Asn-Thr-Cys-Asp-Glu-Lys-Thr-Gln-Ser-Leu-Gly-Val-Lys-Phe-Leu-Asp-Glu -Tyr-Gln-Ser-Lys-Val-Lys-Arg-Gln-Ile-Phe-Ser-Gly-Tyr-Gln-Ser-Ile-Asp-Asp-Thr- His-Asn-Arg-Ile-Lys-Asn-Glu-Leu-COOH. This amino acid sequence was established in part by automated sequence analysis of intact gamma chain as well as of clostripain fragments and tryptic fragments generated from succinylated gamma chain. Smaller peptides from chymotryptic and Staphylococcus aureus protease hydrolyses provided the necessary overlaps. The gamma chain represents a segment of 46 amino acid residues (Mr = 5,395) from the carboxyl-terminal region of the precursor single-chain A subunit of cholera toxin (Duffy, L. K., Peterson, J. W., and Kurosky, A. (1981) FEBS Lett. 126, 187-190). Alignment of the gamma chain region of cholera toxin to a comparable chain region of 36 residues previously reported for heat-labile enterotoxin of Escherichia coli revealed an identity of 31{\%}. The chemical similarity between the gamma chain regions of these two enterotoxins was thus considerably less than that of their beta chains which were shown to be 79{\%} identical.",
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N2 - The complete amino acid sequence of the gamma chain of the A subunit of cholera enterotoxin was determined: NH2-Met-Ser-Asn-Thr-Cys-Asp-Glu-Lys-Thr-Gln-Ser-Leu-Gly-Val-Lys-Phe-Leu-Asp-Glu -Tyr-Gln-Ser-Lys-Val-Lys-Arg-Gln-Ile-Phe-Ser-Gly-Tyr-Gln-Ser-Ile-Asp-Asp-Thr- His-Asn-Arg-Ile-Lys-Asn-Glu-Leu-COOH. This amino acid sequence was established in part by automated sequence analysis of intact gamma chain as well as of clostripain fragments and tryptic fragments generated from succinylated gamma chain. Smaller peptides from chymotryptic and Staphylococcus aureus protease hydrolyses provided the necessary overlaps. The gamma chain represents a segment of 46 amino acid residues (Mr = 5,395) from the carboxyl-terminal region of the precursor single-chain A subunit of cholera toxin (Duffy, L. K., Peterson, J. W., and Kurosky, A. (1981) FEBS Lett. 126, 187-190). Alignment of the gamma chain region of cholera toxin to a comparable chain region of 36 residues previously reported for heat-labile enterotoxin of Escherichia coli revealed an identity of 31%. The chemical similarity between the gamma chain regions of these two enterotoxins was thus considerably less than that of their beta chains which were shown to be 79% identical.

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