COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery

Amar S. More, Hye Min Kim, Ru Zhao, Gilson Khang, Tobias Hildebrandt, Christian Bernlöhr, Henri Doods, Dongwon Lee, Seung Hee Lee, Paul M. Vanhoutte, Dongmei Wu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study examined the vascular effects of bradykinin in health and vascular inflammation comparing responses of isolated pig coronary arteries in the absence and presence of endotoxins. Bradykinin induced contractions in lipopolysaccharide-treated, but not untreated, arterial rings without endothelium. The B2-receptor antagonist HOE140, but not the B1-receptor inhibitor SSR240612, blocked these endothelium-independent contractions in response to bradykinin. The bradykinin-induced contractions were blocked by indomethacin, celecoxib, and terbogrel but not valeryl salicylate, AH6809, AL 8810, or RO1138452. They were attenuated by N-(p-amylcinnamoyl) anthranilic acid, and by diethyldithiocarbamate plus tiron but not by L-NAME. Quantitative reverse-transcription polymerase chain reaction revealed significant upregulations of messenger RNA expressions of B1 receptors, COX-2, and thromboxane A synthase 1 (TBXAS1) following lipopolysaccharide incubation but not of B2 receptors or COX-1. The present data demonstrate that bradykinin induces contractions mediated by the COX-2 pathway in endotoxin-treated pig coronary arteries. These results support differential roles of bradykinin in health and disease.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalJournal of Cardiovascular Pharmacology
Volume64
Issue number3
StatePublished - Sep 1 2014
Externally publishedYes

Fingerprint

Bradykinin
Endotoxins
Endothelium
Coronary Vessels
Swine
Celecoxib
Blood Vessels
Lipopolysaccharides
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Thromboxane-A Synthase
Ditiocarb
Salicylates
NG-Nitroarginine Methyl Ester
Health
Indomethacin
Reverse Transcription
Up-Regulation
Inflammation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Bradykinin
  • Coronary constriction
  • Cyclooxygenase-2

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

More, A. S., Kim, H. M., Zhao, R., Khang, G., Hildebrandt, T., Bernlöhr, C., ... Wu, D. (2014). COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery. Journal of Cardiovascular Pharmacology, 64(3), 209-217.

COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery. / More, Amar S.; Kim, Hye Min; Zhao, Ru; Khang, Gilson; Hildebrandt, Tobias; Bernlöhr, Christian; Doods, Henri; Lee, Dongwon; Lee, Seung Hee; Vanhoutte, Paul M.; Wu, Dongmei.

In: Journal of Cardiovascular Pharmacology, Vol. 64, No. 3, 01.09.2014, p. 209-217.

Research output: Contribution to journalArticle

More, AS, Kim, HM, Zhao, R, Khang, G, Hildebrandt, T, Bernlöhr, C, Doods, H, Lee, D, Lee, SH, Vanhoutte, PM & Wu, D 2014, 'COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery', Journal of Cardiovascular Pharmacology, vol. 64, no. 3, pp. 209-217.
More, Amar S. ; Kim, Hye Min ; Zhao, Ru ; Khang, Gilson ; Hildebrandt, Tobias ; Bernlöhr, Christian ; Doods, Henri ; Lee, Dongwon ; Lee, Seung Hee ; Vanhoutte, Paul M. ; Wu, Dongmei. / COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery. In: Journal of Cardiovascular Pharmacology. 2014 ; Vol. 64, No. 3. pp. 209-217.
@article{295e148e2852412f8496c8847d67dd4f,
title = "COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery",
abstract = "This study examined the vascular effects of bradykinin in health and vascular inflammation comparing responses of isolated pig coronary arteries in the absence and presence of endotoxins. Bradykinin induced contractions in lipopolysaccharide-treated, but not untreated, arterial rings without endothelium. The B2-receptor antagonist HOE140, but not the B1-receptor inhibitor SSR240612, blocked these endothelium-independent contractions in response to bradykinin. The bradykinin-induced contractions were blocked by indomethacin, celecoxib, and terbogrel but not valeryl salicylate, AH6809, AL 8810, or RO1138452. They were attenuated by N-(p-amylcinnamoyl) anthranilic acid, and by diethyldithiocarbamate plus tiron but not by L-NAME. Quantitative reverse-transcription polymerase chain reaction revealed significant upregulations of messenger RNA expressions of B1 receptors, COX-2, and thromboxane A synthase 1 (TBXAS1) following lipopolysaccharide incubation but not of B2 receptors or COX-1. The present data demonstrate that bradykinin induces contractions mediated by the COX-2 pathway in endotoxin-treated pig coronary arteries. These results support differential roles of bradykinin in health and disease.",
keywords = "Bradykinin, Coronary constriction, Cyclooxygenase-2",
author = "More, {Amar S.} and Kim, {Hye Min} and Ru Zhao and Gilson Khang and Tobias Hildebrandt and Christian Bernl{\"o}hr and Henri Doods and Dongwon Lee and Lee, {Seung Hee} and Vanhoutte, {Paul M.} and Dongmei Wu",
year = "2014",
month = "9",
day = "1",
language = "English (US)",
volume = "64",
pages = "209--217",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - COX-2 mediated induction of endothelium-independent contraction to bradykinin in endotoxin-treated porcine coronary artery

AU - More, Amar S.

AU - Kim, Hye Min

AU - Zhao, Ru

AU - Khang, Gilson

AU - Hildebrandt, Tobias

AU - Bernlöhr, Christian

AU - Doods, Henri

AU - Lee, Dongwon

AU - Lee, Seung Hee

AU - Vanhoutte, Paul M.

AU - Wu, Dongmei

PY - 2014/9/1

Y1 - 2014/9/1

N2 - This study examined the vascular effects of bradykinin in health and vascular inflammation comparing responses of isolated pig coronary arteries in the absence and presence of endotoxins. Bradykinin induced contractions in lipopolysaccharide-treated, but not untreated, arterial rings without endothelium. The B2-receptor antagonist HOE140, but not the B1-receptor inhibitor SSR240612, blocked these endothelium-independent contractions in response to bradykinin. The bradykinin-induced contractions were blocked by indomethacin, celecoxib, and terbogrel but not valeryl salicylate, AH6809, AL 8810, or RO1138452. They were attenuated by N-(p-amylcinnamoyl) anthranilic acid, and by diethyldithiocarbamate plus tiron but not by L-NAME. Quantitative reverse-transcription polymerase chain reaction revealed significant upregulations of messenger RNA expressions of B1 receptors, COX-2, and thromboxane A synthase 1 (TBXAS1) following lipopolysaccharide incubation but not of B2 receptors or COX-1. The present data demonstrate that bradykinin induces contractions mediated by the COX-2 pathway in endotoxin-treated pig coronary arteries. These results support differential roles of bradykinin in health and disease.

AB - This study examined the vascular effects of bradykinin in health and vascular inflammation comparing responses of isolated pig coronary arteries in the absence and presence of endotoxins. Bradykinin induced contractions in lipopolysaccharide-treated, but not untreated, arterial rings without endothelium. The B2-receptor antagonist HOE140, but not the B1-receptor inhibitor SSR240612, blocked these endothelium-independent contractions in response to bradykinin. The bradykinin-induced contractions were blocked by indomethacin, celecoxib, and terbogrel but not valeryl salicylate, AH6809, AL 8810, or RO1138452. They were attenuated by N-(p-amylcinnamoyl) anthranilic acid, and by diethyldithiocarbamate plus tiron but not by L-NAME. Quantitative reverse-transcription polymerase chain reaction revealed significant upregulations of messenger RNA expressions of B1 receptors, COX-2, and thromboxane A synthase 1 (TBXAS1) following lipopolysaccharide incubation but not of B2 receptors or COX-1. The present data demonstrate that bradykinin induces contractions mediated by the COX-2 pathway in endotoxin-treated pig coronary arteries. These results support differential roles of bradykinin in health and disease.

KW - Bradykinin

KW - Coronary constriction

KW - Cyclooxygenase-2

UR - http://www.scopus.com/inward/record.url?scp=84907685796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907685796&partnerID=8YFLogxK

M3 - Article

VL - 64

SP - 209

EP - 217

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

IS - 3

ER -