Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends

Rosalie P.C. Driessen, He Meng, Suresh Gorle, Rajesh Shahapure, Giovanni Lanzani, U. Deva Priyakumar, Malcolm F. White, Helmut Schiessel, John Van Noort, Remus Th Dame

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Archaeal chromatin proteins share molecular and functional similarities with both bacterial and eukaryotic chromatin proteins. These proteins play an important role in functionally organizing the genomic DNA into a compact nucleoid. Cren7 and Sul7 are two crenarchaeal nucleoid-associated proteins, which are structurally homologous, but not conserved at the sequence level. Co-crystal structures have shown that these two proteins induce a sharp bend on binding to DNA. In this study, we have investigated the architectural properties of these proteins using atomic force microscopy, molecular dynamics simulations and magnetic tweezers. We demonstrate that Cren7 and Sul7 both compact DNA molecules to a similar extent. Using a theoretical model, we quantify the number of individual proteins bound to the DNA as a function of protein concentration and show that forces up to 3.5 pN do not affect this binding. Moreover, we investigate the flexibility of the bending angle induced by Cren7 and Sul7 and show that the protein-DNA complexes differ in flexibility from analogous bacterial and eukaryotic DNA-bending proteins.

Original languageEnglish (US)
Pages (from-to)196-205
Number of pages10
JournalNucleic acids research
Volume41
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

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Chromatin
DNA
Proteins
Archaeal Proteins
Bacterial DNA
Conserved Sequence
Atomic Force Microscopy
Molecular Dynamics Simulation
Theoretical Models

ASJC Scopus subject areas

  • Genetics

Cite this

Driessen, R. P. C., Meng, H., Gorle, S., Shahapure, R., Lanzani, G., Priyakumar, U. D., ... Dame, R. T. (2013). Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends. Nucleic acids research, 41(1), 196-205. https://doi.org/10.1093/nar/gks1053

Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends. / Driessen, Rosalie P.C.; Meng, He; Gorle, Suresh; Shahapure, Rajesh; Lanzani, Giovanni; Priyakumar, U. Deva; White, Malcolm F.; Schiessel, Helmut; Van Noort, John; Dame, Remus Th.

In: Nucleic acids research, Vol. 41, No. 1, 01.01.2013, p. 196-205.

Research output: Contribution to journalArticle

Driessen, RPC, Meng, H, Gorle, S, Shahapure, R, Lanzani, G, Priyakumar, UD, White, MF, Schiessel, H, Van Noort, J & Dame, RT 2013, 'Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends', Nucleic acids research, vol. 41, no. 1, pp. 196-205. https://doi.org/10.1093/nar/gks1053
Driessen, Rosalie P.C. ; Meng, He ; Gorle, Suresh ; Shahapure, Rajesh ; Lanzani, Giovanni ; Priyakumar, U. Deva ; White, Malcolm F. ; Schiessel, Helmut ; Van Noort, John ; Dame, Remus Th. / Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends. In: Nucleic acids research. 2013 ; Vol. 41, No. 1. pp. 196-205.
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