Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice

Jeroen Kortekaas, Rianka P M Vloet, Alexander J. Mcauley, Xiaoli Shen, Berend Jan Bosch, Laura De Vries, Rob J M Moormann, Dennis Bente

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Crimean-Congo hemorrhagic fever virus is a tick-borne bunyavirus of the Nairovirus genus that causes hemorrhagic fever in humans with high case fatality. Here, we report the development of subunit vaccines and their efficacy in signal transducer and activator of transcription 1 (STAT1) knockout mice. Ectodomains of the structural glycoproteins Gn and Gc were produced using a Drosophila insect cell-based expression system. A single vaccination of STAT129 mice with adjuvanted Gn or Gc ectodomains induced neutralizing antibody responses, which were boosted by a second vaccination. Despite these antibody responses, mice were not protected from a CCHFV challenge infection. These results suggest that neutralizing antibodies against CCHFV do not correlate with protection of STAT1 knockout mice.

Original languageEnglish (US)
Pages (from-to)759-764
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume15
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Crimean-Congo hemorrhagic fever virus
STAT1 Transcription Factor
Subunit Vaccines
Neutralizing Antibodies
Knockout Mice
Antibody Formation
Nairovirus
Vaccination
Orthobunyavirus
Ticks
Drosophila
Insects
Fever
Infection

Keywords

  • Bunyavirus
  • Crimean-Congo hemorrhagic fever virus
  • Gc
  • Gn
  • Nairovirus
  • Neutralizing antibodies
  • STAT1
  • STAT129
  • Vaccine

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology

Cite this

Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice. / Kortekaas, Jeroen; Vloet, Rianka P M; Mcauley, Alexander J.; Shen, Xiaoli; Bosch, Berend Jan; De Vries, Laura; Moormann, Rob J M; Bente, Dennis.

In: Vector-Borne and Zoonotic Diseases, Vol. 15, No. 12, 01.12.2015, p. 759-764.

Research output: Contribution to journalArticle

Kortekaas, Jeroen ; Vloet, Rianka P M ; Mcauley, Alexander J. ; Shen, Xiaoli ; Bosch, Berend Jan ; De Vries, Laura ; Moormann, Rob J M ; Bente, Dennis. / Crimean-congo hemorrhagic fever virus subunit vaccines induce high levels of neutralizing antibodies but no protection in STAT1 knockout mice. In: Vector-Borne and Zoonotic Diseases. 2015 ; Vol. 15, No. 12. pp. 759-764.
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