Cross-Reaction of Two Different Somatostatin Antisera with Calcitonin Gene-Related Peptide

Jin Ishizuka; George H. Greeley; James C. Thompson; Chizuko Yanaihara; Noboru Yanaihara; Cary W. Cooper

doi:10.3181/00379727-189-42771

Cross-Reaction of Two Different Somatostatin Antisera with Calcitonin Gene-Related Peptide

Jin Ishizuka, George H. Greeley, James C. Thompson, Chizuko Yanaihara, Noboru Yanaihara, Cary W. Cooper

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Two different anti-somatostatin antisera, R-101 and OAL-273, cross-react with rat calcitonin gene-related peptide (1–37) (CGRP). CGRP amounts, in excess of 6.25 × 10^-9M, cross-react with R-101 in the somatostatin radioimmunoassay. CGRP amounts, in excess of 1.6 × 10^-9M, cross-react with OAL-273. Both CGRP displacement curves are parallel to that of synthetic somatostatin (1–14). Comparison of ID₅₀'s shows that the cross-reactivity of CGRP with R-101 and OAL-273 are 0.02 and 0.1% of somatostatin, respectively.

Original language	English (US)
Pages (from-to)	1-4
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	189
Issue number	1
DOIs	https://doi.org/10.3181/00379727-189-42771
State	Published - Oct 1988
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Cross-Reaction of Two Different Somatostatin Antisera with Calcitonin Gene-Related Peptide",

abstract = "Two different anti-somatostatin antisera, R-101 and OAL-273, cross-react with rat calcitonin gene-related peptide (1–37) (CGRP). CGRP amounts, in excess of 6.25 × 10-9M, cross-react with R-101 in the somatostatin radioimmunoassay. CGRP amounts, in excess of 1.6 × 10-9M, cross-react with OAL-273. Both CGRP displacement curves are parallel to that of synthetic somatostatin (1–14). Comparison of ID50's shows that the cross-reactivity of CGRP with R-101 and OAL-273 are 0.02 and 0.1% of somatostatin, respectively.",

author = "Jin Ishizuka and Greeley, {George H.} and Thompson, {James C.} and Chizuko Yanaihara and Noboru Yanaihara and Cooper, {Cary W.}",

note = "Funding Information: Supported by grants from the National Institutes of Health (RO1 DK 15241, PO1 DK 35608).",

year = "1988",

month = oct,

doi = "10.3181/00379727-189-42771",

language = "English (US)",

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T1 - Cross-Reaction of Two Different Somatostatin Antisera with Calcitonin Gene-Related Peptide

AU - Ishizuka, Jin

AU - Greeley, George H.

AU - Thompson, James C.

AU - Yanaihara, Chizuko

AU - Yanaihara, Noboru

AU - Cooper, Cary W.

N1 - Funding Information: Supported by grants from the National Institutes of Health (RO1 DK 15241, PO1 DK 35608).

PY - 1988/10

Y1 - 1988/10

N2 - Two different anti-somatostatin antisera, R-101 and OAL-273, cross-react with rat calcitonin gene-related peptide (1–37) (CGRP). CGRP amounts, in excess of 6.25 × 10-9M, cross-react with R-101 in the somatostatin radioimmunoassay. CGRP amounts, in excess of 1.6 × 10-9M, cross-react with OAL-273. Both CGRP displacement curves are parallel to that of synthetic somatostatin (1–14). Comparison of ID50's shows that the cross-reactivity of CGRP with R-101 and OAL-273 are 0.02 and 0.1% of somatostatin, respectively.

AB - Two different anti-somatostatin antisera, R-101 and OAL-273, cross-react with rat calcitonin gene-related peptide (1–37) (CGRP). CGRP amounts, in excess of 6.25 × 10-9M, cross-react with R-101 in the somatostatin radioimmunoassay. CGRP amounts, in excess of 1.6 × 10-9M, cross-react with OAL-273. Both CGRP displacement curves are parallel to that of synthetic somatostatin (1–14). Comparison of ID50's shows that the cross-reactivity of CGRP with R-101 and OAL-273 are 0.02 and 0.1% of somatostatin, respectively.

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Cross-Reaction of Two Different Somatostatin Antisera with Calcitonin Gene-Related Peptide

Abstract

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