Crucial role of endogenous interleukin-10 production in myocardial ischemia/reperfusion injury

Zequan Yang, Basilia Zingarelli, Csaba Szabo

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

Background - The anti-inflammatory cytokine interleukin-10 (IL-10) has been detected in the plasma of patients with myocardial ischemia/reperfusion. The aim of our study was to investigate the role of endogenously produced IL-10 in myocardial ischemia/reperfusion. Methods and Results - In the present study, we used wild-type and IL-10-deficient mice subjected to myocardial ischemia/reperfusion. Significant levels of IL-10 were produced in wild-type mice at 2 to 6 hours after myocardial reperfusion. The genetic deletion of IL-10 enhanced neutrophil infiltration into the reperfused tissues at 6 hours after reperfusion and increased infarct size and myocardial necrosis. Furthermore, in the absence of IL-10, an enhancement of the inflammatory response was seen, as demonstrated by increased plasma levels of tumor necrosis factor-α, nitrite/nitrate (breakdown products of NO), and increased tissue expression of intercellular adhesion molecule-1. Reperfusion for 24 hours was associated with a 75% mortality rate in IL-10-deficient mice, whereas no deaths occurred in the wild-type animals. Conclusions -The present findings provide the first direct evidence that endogenous IL-10 inhibits the production of tumor necrosis factor-α and NO and serves to protect the ischemic and reperfused myocardium through the suppression of neutrophil recruitment.

Original languageEnglish (US)
Pages (from-to)1019-1026
Number of pages8
JournalCirculation
Volume101
Issue number9
StatePublished - Mar 7 2000
Externally publishedYes

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Myocardial Reperfusion Injury
Reperfusion Injury
Interleukin-10
Myocardial Ischemia
Myocardial Reperfusion
Neutrophil Infiltration
Reperfusion
Tumor Necrosis Factor-alpha
Wild Animals
Intercellular Adhesion Molecule-1
Nitrites
Nitrates
Myocardium
Anti-Inflammatory Agents
Necrosis
Myocardial Infarction
Cytokines
Mortality

Keywords

  • Cell adhesion molecules
  • Interleukins
  • Nitric oxide
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Crucial role of endogenous interleukin-10 production in myocardial ischemia/reperfusion injury. / Yang, Zequan; Zingarelli, Basilia; Szabo, Csaba.

In: Circulation, Vol. 101, No. 9, 07.03.2000, p. 1019-1026.

Research output: Contribution to journalArticle

Yang, Zequan ; Zingarelli, Basilia ; Szabo, Csaba. / Crucial role of endogenous interleukin-10 production in myocardial ischemia/reperfusion injury. In: Circulation. 2000 ; Vol. 101, No. 9. pp. 1019-1026.
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N2 - Background - The anti-inflammatory cytokine interleukin-10 (IL-10) has been detected in the plasma of patients with myocardial ischemia/reperfusion. The aim of our study was to investigate the role of endogenously produced IL-10 in myocardial ischemia/reperfusion. Methods and Results - In the present study, we used wild-type and IL-10-deficient mice subjected to myocardial ischemia/reperfusion. Significant levels of IL-10 were produced in wild-type mice at 2 to 6 hours after myocardial reperfusion. The genetic deletion of IL-10 enhanced neutrophil infiltration into the reperfused tissues at 6 hours after reperfusion and increased infarct size and myocardial necrosis. Furthermore, in the absence of IL-10, an enhancement of the inflammatory response was seen, as demonstrated by increased plasma levels of tumor necrosis factor-α, nitrite/nitrate (breakdown products of NO), and increased tissue expression of intercellular adhesion molecule-1. Reperfusion for 24 hours was associated with a 75% mortality rate in IL-10-deficient mice, whereas no deaths occurred in the wild-type animals. Conclusions -The present findings provide the first direct evidence that endogenous IL-10 inhibits the production of tumor necrosis factor-α and NO and serves to protect the ischemic and reperfused myocardium through the suppression of neutrophil recruitment.

AB - Background - The anti-inflammatory cytokine interleukin-10 (IL-10) has been detected in the plasma of patients with myocardial ischemia/reperfusion. The aim of our study was to investigate the role of endogenously produced IL-10 in myocardial ischemia/reperfusion. Methods and Results - In the present study, we used wild-type and IL-10-deficient mice subjected to myocardial ischemia/reperfusion. Significant levels of IL-10 were produced in wild-type mice at 2 to 6 hours after myocardial reperfusion. The genetic deletion of IL-10 enhanced neutrophil infiltration into the reperfused tissues at 6 hours after reperfusion and increased infarct size and myocardial necrosis. Furthermore, in the absence of IL-10, an enhancement of the inflammatory response was seen, as demonstrated by increased plasma levels of tumor necrosis factor-α, nitrite/nitrate (breakdown products of NO), and increased tissue expression of intercellular adhesion molecule-1. Reperfusion for 24 hours was associated with a 75% mortality rate in IL-10-deficient mice, whereas no deaths occurred in the wild-type animals. Conclusions -The present findings provide the first direct evidence that endogenous IL-10 inhibits the production of tumor necrosis factor-α and NO and serves to protect the ischemic and reperfused myocardium through the suppression of neutrophil recruitment.

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