Cryo-electron crystallography of small and mosaic 2-D crystals

An assessment of a procedure for high-resolution data retrieval

Svetla S. Stoylova, Robert C. Ford, Andreas Holzenburg

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We describe a procedure to obtain structural data of biological macromolecules from small 2-D crystals using cryo-electron crystallography. The procedure has been applied to a membrane-embedded multi-protein complex (photosystem II). This complex, under certain conditions, forms 2-D crystals in its native membrane which are composed of many small mosaic patches and therefore represents an ideal test specimen. By averaging in reciprocal space over more than 50 crystalline patches, it was possible to calculate a projection map using data approaching 1.3 nm resolution while in the Fourier transforms of individual patches only reflections up to the 4th order were readily identifiable. The efficiency of the procedure was evaluated by examining the fidelity of (i) the rotational alignment of patches prior to merging, (ii) the space group assignment, (iii) phase and amplitude extraction, and (iv) the correction of the structure factors for the effects of the contrast transfer function (CTF).

Original languageEnglish (US)
Pages (from-to)113-128
Number of pages16
JournalUltramicroscopy
Volume77
Issue number3-4
DOIs
StatePublished - Jul 1999
Externally publishedYes

Fingerprint

data retrieval
Crystallography
crystallography
membranes
Membranes
Crystals
Photosystem II Protein Complex
Electrons
high resolution
Macromolecules
Merging
macromolecules
transfer functions
crystals
Transfer functions
Fourier transforms
electrons
projection
alignment
Crystalline materials

Keywords

  • Cryo-electron crystallography
  • Image processing
  • Mosaicity
  • Small 2-D crystals

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation

Cite this

Cryo-electron crystallography of small and mosaic 2-D crystals : An assessment of a procedure for high-resolution data retrieval. / Stoylova, Svetla S.; Ford, Robert C.; Holzenburg, Andreas.

In: Ultramicroscopy, Vol. 77, No. 3-4, 07.1999, p. 113-128.

Research output: Contribution to journalArticle

Stoylova, Svetla S. ; Ford, Robert C. ; Holzenburg, Andreas. / Cryo-electron crystallography of small and mosaic 2-D crystals : An assessment of a procedure for high-resolution data retrieval. In: Ultramicroscopy. 1999 ; Vol. 77, No. 3-4. pp. 113-128.
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