Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage φ29

Marc Morais, Yizhi Tao, Norman H. Olson, Shelley Grimes, Paul J. Jardine, Dwight L. Anderson, Timothy S. Baker, Michael G. Rossmann

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

A method has been developed for three-dimensional image reconstruction of symmetry-mismatched components in tailed phages. Although the method described here addresses the specific case where differing symmetry axes are coincident, the method is more generally applicable, for instance, to the reconstruction of images of viral particles that deviate from icosahedral symmetry. Particles are initially oriented according to their dominant symmetry, thus reducing the search space for determining the orientation of the less dominant, symmetry-mismatched component. This procedure produced an improved reconstruction of the sixfold-symmetric tail assembly that is attached to the fivefold-symmetric prolate head of φ29, demonstrating that this method is capable of detecting and reconstructing an object that included a symmetry mismatch. A reconstruction of φ29 prohead particles using the methods described here establishes that the pRNA molecule has fivefold symmetry when attached to the prohead, consistent with its proposed role as a component of the stator in the φ29 DNA packaging motor.

Original languageEnglish (US)
Pages (from-to)38-46
Number of pages9
JournalJournal of Structural Biology
Volume135
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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Cryoelectron Microscopy
Computer-Assisted Image Processing
Bacteriophages
Phosmet
DNA Packaging
Three-Dimensional Imaging
Virion

Keywords

  • φ29
  • Cryo-EM image reconstruction
  • pRNA
  • Symmetry mismatch
  • Tail appendages

ASJC Scopus subject areas

  • Structural Biology

Cite this

Morais, M., Tao, Y., Olson, N. H., Grimes, S., Jardine, P. J., Anderson, D. L., ... Rossmann, M. G. (2001). Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage φ29. Journal of Structural Biology, 135(1), 38-46. https://doi.org/10.1006/jsbi.2001.4379

Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage φ29. / Morais, Marc; Tao, Yizhi; Olson, Norman H.; Grimes, Shelley; Jardine, Paul J.; Anderson, Dwight L.; Baker, Timothy S.; Rossmann, Michael G.

In: Journal of Structural Biology, Vol. 135, No. 1, 2001, p. 38-46.

Research output: Contribution to journalArticle

Morais, M, Tao, Y, Olson, NH, Grimes, S, Jardine, PJ, Anderson, DL, Baker, TS & Rossmann, MG 2001, 'Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage φ29', Journal of Structural Biology, vol. 135, no. 1, pp. 38-46. https://doi.org/10.1006/jsbi.2001.4379
Morais, Marc ; Tao, Yizhi ; Olson, Norman H. ; Grimes, Shelley ; Jardine, Paul J. ; Anderson, Dwight L. ; Baker, Timothy S. ; Rossmann, Michael G. / Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage φ29. In: Journal of Structural Biology. 2001 ; Vol. 135, No. 1. pp. 38-46.
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