Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Samantha Nava; Aygul Sadiqova; Alejandro Castellanos-Gonzalez; A. Clinton White

doi:10.1016/j.molbiopara.2020.111277

Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Samantha Nava, Aygul Sadiqova, Alejandro Castellanos-Gonzalez, A. Clinton White

Internal Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.

Original language	English (US)
Article number	111277
Journal	Molecular and Biochemical Parasitology
Volume	237
DOIs	https://doi.org/10.1016/j.molbiopara.2020.111277
State	Published - May 2020

Keywords

Cryptosporidium
Cyclic GMP-dependent protein kinase
Egress

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/j.molbiopara.2020.111277

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title = "Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress",

abstract = "Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.",

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T2 - An essential mediator of merozoite egress

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AU - Sadiqova, Aygul

AU - Castellanos-Gonzalez, Alejandro

AU - White, A. Clinton

PY - 2020/5

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AB - Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.

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Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress

Abstract

Keywords

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