TY - JOUR
T1 - Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG)
T2 - An essential mediator of merozoite egress
AU - Nava, Samantha
AU - Sadiqova, Aygul
AU - Castellanos-Gonzalez, Alejandro
AU - White, A. Clinton
N1 - Funding Information:
S.N. was supported by a fellowship from the Sealy Center for Vaccine Development at the University of Texas Medical Branch at Galveston. This project was supported by the Bill & Melinda Gates Foundation (Grant No.: OPP1161026 ) and 5R21AI12627502 by the National Institute of Allergy and Infectious Diseases , National Institute of Health .
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/5
Y1 - 2020/5
N2 - Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.
AB - Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.
KW - Cryptosporidium
KW - Cyclic GMP-dependent protein kinase
KW - Egress
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U2 - 10.1016/j.molbiopara.2020.111277
DO - 10.1016/j.molbiopara.2020.111277
M3 - Article
C2 - 32348840
AN - SCOPUS:85084346223
SN - 0166-6851
VL - 237
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
M1 - 111277
ER -