Crystal structure and location of gp131 in the bacteriophage phiKZ virion

Lada V. Sycheva, Mikhail M. Shneider, Nina N. Sykilinda, Maria A. Ivanova, Konstantin A. Miroshnikov, Petr Leiman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pseudomonas phage ΦKZ and its two close relatives ΦPA3 and 201Φ2-1 are very large bacteriophages that form a separate branch in phage classification because their genomes are very different from the rest of GenBank sequence data. The contractile tail of ΦKZ is built from at least 32 different proteins, but a definitive structural function is assigned to only one of them-the tail sheath protein. Here, we report the crystal structure of the C-terminal domain of another phiKZ tail protein, gene product 131 (gp131C). We show that gp131 is located at the periphery of the baseplate and possibly associates with fibers that emanate from the baseplate. Gp131C is a seven-bladed Β-propeller that has a shape of a skewed toroid. A small but highly conserved and negatively charged patch on the surface of gp131C might be important for substrate binding or for interaction with a different tail protein.

Original languageEnglish (US)
Pages (from-to)257-264
Number of pages8
JournalVirology
Volume434
Issue number2
DOIs
StatePublished - Dec 20 2012
Externally publishedYes

Fingerprint

Virion
Bacteriophages
Pseudomonas Phages
Proteins
Nucleic Acid Databases
Genome

Keywords

  • ImmunoEM
  • Phage protein
  • Seven-bladed beta-propeller
  • Virion component
  • X-ray crystallography

ASJC Scopus subject areas

  • Medicine(all)
  • Virology

Cite this

Sycheva, L. V., Shneider, M. M., Sykilinda, N. N., Ivanova, M. A., Miroshnikov, K. A., & Leiman, P. (2012). Crystal structure and location of gp131 in the bacteriophage phiKZ virion. Virology, 434(2), 257-264. https://doi.org/10.1016/j.virol.2012.09.001

Crystal structure and location of gp131 in the bacteriophage phiKZ virion. / Sycheva, Lada V.; Shneider, Mikhail M.; Sykilinda, Nina N.; Ivanova, Maria A.; Miroshnikov, Konstantin A.; Leiman, Petr.

In: Virology, Vol. 434, No. 2, 20.12.2012, p. 257-264.

Research output: Contribution to journalArticle

Sycheva, LV, Shneider, MM, Sykilinda, NN, Ivanova, MA, Miroshnikov, KA & Leiman, P 2012, 'Crystal structure and location of gp131 in the bacteriophage phiKZ virion', Virology, vol. 434, no. 2, pp. 257-264. https://doi.org/10.1016/j.virol.2012.09.001
Sycheva LV, Shneider MM, Sykilinda NN, Ivanova MA, Miroshnikov KA, Leiman P. Crystal structure and location of gp131 in the bacteriophage phiKZ virion. Virology. 2012 Dec 20;434(2):257-264. https://doi.org/10.1016/j.virol.2012.09.001
Sycheva, Lada V. ; Shneider, Mikhail M. ; Sykilinda, Nina N. ; Ivanova, Maria A. ; Miroshnikov, Konstantin A. ; Leiman, Petr. / Crystal structure and location of gp131 in the bacteriophage phiKZ virion. In: Virology. 2012 ; Vol. 434, No. 2. pp. 257-264.
@article{43a6600df1de44989c0b2ac309cda8b5,
title = "Crystal structure and location of gp131 in the bacteriophage phiKZ virion",
abstract = "Pseudomonas phage ΦKZ and its two close relatives ΦPA3 and 201Φ2-1 are very large bacteriophages that form a separate branch in phage classification because their genomes are very different from the rest of GenBank sequence data. The contractile tail of ΦKZ is built from at least 32 different proteins, but a definitive structural function is assigned to only one of them-the tail sheath protein. Here, we report the crystal structure of the C-terminal domain of another phiKZ tail protein, gene product 131 (gp131C). We show that gp131 is located at the periphery of the baseplate and possibly associates with fibers that emanate from the baseplate. Gp131C is a seven-bladed Β-propeller that has a shape of a skewed toroid. A small but highly conserved and negatively charged patch on the surface of gp131C might be important for substrate binding or for interaction with a different tail protein.",
keywords = "ImmunoEM, Phage protein, Seven-bladed beta-propeller, Virion component, X-ray crystallography",
author = "Sycheva, {Lada V.} and Shneider, {Mikhail M.} and Sykilinda, {Nina N.} and Ivanova, {Maria A.} and Miroshnikov, {Konstantin A.} and Petr Leiman",
year = "2012",
month = "12",
day = "20",
doi = "10.1016/j.virol.2012.09.001",
language = "English (US)",
volume = "434",
pages = "257--264",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Crystal structure and location of gp131 in the bacteriophage phiKZ virion

AU - Sycheva, Lada V.

AU - Shneider, Mikhail M.

AU - Sykilinda, Nina N.

AU - Ivanova, Maria A.

AU - Miroshnikov, Konstantin A.

AU - Leiman, Petr

PY - 2012/12/20

Y1 - 2012/12/20

N2 - Pseudomonas phage ΦKZ and its two close relatives ΦPA3 and 201Φ2-1 are very large bacteriophages that form a separate branch in phage classification because their genomes are very different from the rest of GenBank sequence data. The contractile tail of ΦKZ is built from at least 32 different proteins, but a definitive structural function is assigned to only one of them-the tail sheath protein. Here, we report the crystal structure of the C-terminal domain of another phiKZ tail protein, gene product 131 (gp131C). We show that gp131 is located at the periphery of the baseplate and possibly associates with fibers that emanate from the baseplate. Gp131C is a seven-bladed Β-propeller that has a shape of a skewed toroid. A small but highly conserved and negatively charged patch on the surface of gp131C might be important for substrate binding or for interaction with a different tail protein.

AB - Pseudomonas phage ΦKZ and its two close relatives ΦPA3 and 201Φ2-1 are very large bacteriophages that form a separate branch in phage classification because their genomes are very different from the rest of GenBank sequence data. The contractile tail of ΦKZ is built from at least 32 different proteins, but a definitive structural function is assigned to only one of them-the tail sheath protein. Here, we report the crystal structure of the C-terminal domain of another phiKZ tail protein, gene product 131 (gp131C). We show that gp131 is located at the periphery of the baseplate and possibly associates with fibers that emanate from the baseplate. Gp131C is a seven-bladed Β-propeller that has a shape of a skewed toroid. A small but highly conserved and negatively charged patch on the surface of gp131C might be important for substrate binding or for interaction with a different tail protein.

KW - ImmunoEM

KW - Phage protein

KW - Seven-bladed beta-propeller

KW - Virion component

KW - X-ray crystallography

UR - http://www.scopus.com/inward/record.url?scp=84870686581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870686581&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2012.09.001

DO - 10.1016/j.virol.2012.09.001

M3 - Article

C2 - 23031178

AN - SCOPUS:84870686581

VL - 434

SP - 257

EP - 264

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -