Crystal structure of cone arrestin at 2.3 Å: Evolution of receptor specificity

R. Bryan Sutton, Sergey A. Vishnivetskiy, Justin Robert, Susan M. Hanson, Dayanidhi Raman, Barry E. Knox, Masahiro Kono, Javier Navarro, Vsevolod V. Gurevich

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Arrestins play a fundamental role in the regulation and signal transduction of G protein-coupled receptors. Here we describe the crystal structure of cone arrestin at 2.3 Å resolution. The overall structure of cone visual arrestin is similar to the crystal structures of rod visual and the non-visual arrestin-2, consisting of two domains, each containing ten β-sheets. However, at the tertiary structure level, there are two major differences, in particular on the concave surfaces of the two domains implicated in receptor binding and in the loop between β-strands I and II. Functional analysis shows that cone arrestin, in sharp contrast to its rod counterpart, bound cone pigments and non-visual receptors. Conversely, non-visual arrestin-2 bound cone pigments, suggesting that it may also regulate phototransduction and/or photopigment trafficking in cone photoreceptors. These findings indicate that cone arrestin displays structural and functional features intermediate between the specialized rod arrestin and the non-visual arrestins, which have broad receptor specificity. A unique functional feature of cone arrestin was the low affinity for its cognate receptor, resulting in an unusually rapid dissociation of the complex. Transient arrestin binding to the photopigment in cones may be responsible for the extremely rapid regeneration and reuse of the photopigment that is essential for cone function at high levels of illumination.

Original languageEnglish (US)
Pages (from-to)1069-1080
Number of pages12
JournalJournal of Molecular Biology
Volume354
Issue number5
DOIs
StatePublished - Dec 16 2005

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Keywords

  • Cone arrestin
  • G protein-coupled receptors
  • Signal transduction
  • Vision
  • X-ray structure

ASJC Scopus subject areas

  • Virology

Cite this

Sutton, R. B., Vishnivetskiy, S. A., Robert, J., Hanson, S. M., Raman, D., Knox, B. E., Kono, M., Navarro, J., & Gurevich, V. V. (2005). Crystal structure of cone arrestin at 2.3 Å: Evolution of receptor specificity. Journal of Molecular Biology, 354(5), 1069-1080. https://doi.org/10.1016/j.jmb.2005.10.023