Crystal structure of the Dengue virus methyltransferase bound to a 5′-capped octameric RNA

Li Jian Yap, Dahai Luo, Ka Yan Chung, Siew Pheng Lim, Christophe Bodenreider, Christian Noble, Pei-Yong Shi, Julien Lescar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The N-terminal domain of the flavivirus NS5 protein functions as a methyltransferase (MTase). It sequentially methylates the N7 and 2′-O positions of the viral RNA cap structure (GpppA→7meGpppA→7meGpppA2′-O- me). The same NS5 domain could also have a guanylyltransferase activity (GTP+ppA-RNA→GpppA). The mechanism by which this protein domain catalyzes these three distinct functions is currently unknown. Here we report the crystallographic structure of DENV-3 MTase in complex with a 5′-capped RNA octamer (GpppAGAACCUG) at a resolution of 2.9 Å. Two RNA octamers arranged as kissing loops are encircled by four MTase monomers around a 2-fold non-crystallography symmetry axis. Only two of the four monomers make direct contact with the 5′ end of RNA. The RNA structure is stabilised by the formation of several intra and intermolecular base stacking and non-canonical base pairs. The structure may represent the product of guanylylation of the viral genome prior to the subsequent methylation events that require repositioning of the RNA substrate to reach to the methyl-donor sites. The crystal structure provides a structural explanation for the observed trans-complementation of MTases with different methylation defects.

Original languageEnglish (US)
Article numbere12836
Pages (from-to)1-9
Number of pages9
JournalPLoS One
Volume5
Issue number9
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

RNA Caps
Dengue virus
Dengue Virus
methyltransferases
Methyltransferases
crystal structure
Viruses
Crystal structure
RNA
Methylation
Monomers
methylation
Viral Genome
Viral RNA
Guanosine Triphosphate
Base Pairing
Flavivirus
Genes
direct contact
Defects

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yap, L. J., Luo, D., Chung, K. Y., Lim, S. P., Bodenreider, C., Noble, C., ... Lescar, J. (2010). Crystal structure of the Dengue virus methyltransferase bound to a 5′-capped octameric RNA. PLoS One, 5(9), 1-9. [e12836]. https://doi.org/10.1371/journal.pone.0012836

Crystal structure of the Dengue virus methyltransferase bound to a 5′-capped octameric RNA. / Yap, Li Jian; Luo, Dahai; Chung, Ka Yan; Lim, Siew Pheng; Bodenreider, Christophe; Noble, Christian; Shi, Pei-Yong; Lescar, Julien.

In: PLoS One, Vol. 5, No. 9, e12836, 2010, p. 1-9.

Research output: Contribution to journalArticle

Yap, LJ, Luo, D, Chung, KY, Lim, SP, Bodenreider, C, Noble, C, Shi, P-Y & Lescar, J 2010, 'Crystal structure of the Dengue virus methyltransferase bound to a 5′-capped octameric RNA', PLoS One, vol. 5, no. 9, e12836, pp. 1-9. https://doi.org/10.1371/journal.pone.0012836
Yap, Li Jian ; Luo, Dahai ; Chung, Ka Yan ; Lim, Siew Pheng ; Bodenreider, Christophe ; Noble, Christian ; Shi, Pei-Yong ; Lescar, Julien. / Crystal structure of the Dengue virus methyltransferase bound to a 5′-capped octameric RNA. In: PLoS One. 2010 ; Vol. 5, No. 9. pp. 1-9.
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