Crystallization and characterization of bovine liver glutamate dehydrogenase

Peter E. Peterson, Jonathan Pierce, Thomas Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Bovine liver glutamate dehydrogenase has been crystallized as an abortive complex with glutamic acid, NADH, and an inhibitor, GTP. Crystals of this complex were grown using the sitting drop vapor diffusion method with PEG 8000 as the precipitant and diffract to better than 2.5 Å resolution. The crystals belong to the space group P21 with an entire enzyme hexamer in the crystallographic asymmetric unit. Self-rotation and self-Patterson functions clearly define the orientation and position of this hexameric enzyme.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalJournal of Structural Biology
Volume120
Issue number1
DOIs
StatePublished - Oct 1997
Externally publishedYes

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Glutamate Dehydrogenase
Crystallization
Liver
Enzymes
Guanosine Triphosphate
NAD
Glutamic Acid
polyethylene glycol 8000

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystallization and characterization of bovine liver glutamate dehydrogenase. / Peterson, Peter E.; Pierce, Jonathan; Smith, Thomas.

In: Journal of Structural Biology, Vol. 120, No. 1, 10.1997, p. 73-77.

Research output: Contribution to journalArticle

Peterson, Peter E. ; Pierce, Jonathan ; Smith, Thomas. / Crystallization and characterization of bovine liver glutamate dehydrogenase. In: Journal of Structural Biology. 1997 ; Vol. 120, No. 1. pp. 73-77.
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