Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei

Dequin Liu, Terumi Midoro-Horiuti, Mark A. White, Edward G. Brooks, Randall M. Goldblum, Edmund W. Czerwinski

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Jun a 1, the major allergen of pollen from the mountain cedar Juniperus ashei, has been crystallized using the hanging-drop vapor-diffusion method at 277 K. The crystals are monoclinic, space group P21, with unit-cell parameters a = 53.38, b = 113.48, c = 72.44 Å, β = 96.36° and four molecules in the unit cell. A complete 2.5 Å data set has been collected at 100 K with X-rays from a Cu Kα rotatinganode generator.

Original languageEnglish (US)
Pages (from-to)1052-1054
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Issue number6
StatePublished - Jun 1 2003


ASJC Scopus subject areas

  • Structural Biology

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