Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei

Dequin Liu, Terumi Midoro-Horiuti, Mark White, Edward G. Brooks, Randall M. Goldblum, Edmund W. Czerwinski

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Jun a 1, the major allergen of pollen from the mountain cedar Juniperus ashei, has been crystallized using the hanging-drop vapor-diffusion method at 277 K. The crystals are monoclinic, space group P21, with unit-cell parameters a = 53.38, b = 113.48, c = 72.44 Å, β = 96.36° and four molecules in the unit cell. A complete 2.5 Å data set has been collected at 100 K with X-rays from a Cu Kα rotatinganode generator.

Original languageEnglish (US)
Pages (from-to)1052-1054
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number6
DOIs
StatePublished - Jun 1 2003

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Juniperus
pollen
Pollen
Crystallization
mountains
X-Ray Diffraction
Allergens
X ray diffraction analysis
Vapors
crystallization
X rays
Crystals
Molecules
cells
diffraction
x rays
generators
X-Rays
vapors
crystals

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei. / Liu, Dequin; Midoro-Horiuti, Terumi; White, Mark; Brooks, Edward G.; Goldblum, Randall M.; Czerwinski, Edmund W.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 6, 01.06.2003, p. 1052-1054.

Research output: Contribution to journalArticle

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