Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum

Yasuhiro Higashi, Thomas Smith, Joseph M. Jez, Toni M. Kutchan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6422 or P6222 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 Å.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number2
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Papaver
Opium
morphine
Crystallization
X-Ray Diffraction
X ray diffraction analysis
Morphine
Oxidoreductases
crystallization
Codeine
Size exclusion chromatography
Papaver salutaridine reductase
Narcotics
Ammonium Sulfate
Cobalt
narcotics
diffraction
Hydroxyl Radical
Escherichia coli
ammonium sulfates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

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abstract = "The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6422 or P6222 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 {\AA}.",
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T1 - Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum

AU - Higashi, Yasuhiro

AU - Smith, Thomas

AU - Jez, Joseph M.

AU - Kutchan, Toni M.

PY - 2010

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AB - The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6422 or P6222 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 Å.

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