Crystallographic Insights into the Autocatalytic Assembly Mechanism of a Bacteriophage Tail Spike

Ye Xiang, Petr Leiman, Long Li, Shelley Grimes, Dwight L. Anderson, Michael G. Rossmann

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The tailed bacteriophage φ{symbol}29 has 12 "appendages" (gene product 12, gp12) attached to its neck region that participate in host cell recognition and entry. In the cell, monomeric gp12 undergoes proteolytic processing that releases the C-terminal domain during assembly into trimers. We report here crystal structures of the protein before and after catalytic processing and show that the C-terminal domain of gp12 is an "autochaperone" that aids trimerization. We also show that autocleavage of the C-terminal domain is a posttrimerization event that is followed by a unique ATP-dependent release. The posttranslationally modified N-terminal part has three domains that function to attach the appendages to the phage, digest the cell wall teichoic acids, and bind irreversibly to the host, respectively. Structural and sequence comparisons suggest that some eukaryotic and bacterial viruses as well as bacterial adhesins might have a similar maturation mechanism as is performed by φ{symbol}29 gp12 for Bacillus subtilis.

Original languageEnglish (US)
Pages (from-to)375-386
Number of pages12
JournalMolecular Cell
Volume34
Issue number3
DOIs
StatePublished - May 15 2009
Externally publishedYes

Fingerprint

Bacteriophages
Genes
Bacterial Adhesins
Teichoic Acids
Bacillus subtilis
Cell Wall
Neck
Adenosine Triphosphate
Viruses
Proteins

Keywords

  • MICROBIO
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Crystallographic Insights into the Autocatalytic Assembly Mechanism of a Bacteriophage Tail Spike. / Xiang, Ye; Leiman, Petr; Li, Long; Grimes, Shelley; Anderson, Dwight L.; Rossmann, Michael G.

In: Molecular Cell, Vol. 34, No. 3, 15.05.2009, p. 375-386.

Research output: Contribution to journalArticle

Xiang, Ye ; Leiman, Petr ; Li, Long ; Grimes, Shelley ; Anderson, Dwight L. ; Rossmann, Michael G. / Crystallographic Insights into the Autocatalytic Assembly Mechanism of a Bacteriophage Tail Spike. In: Molecular Cell. 2009 ; Vol. 34, No. 3. pp. 375-386.
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