CTF determination of images of ice-embedded single particles using a graphics interface

Z. Hong Zhou, Steve Hardt, Bin Wang, Michael B. Sherman, Joanita Jakana, Wah Chiu

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

We implement a graphical user interface in an X-window/UNIX environment to compute and display the incoherently averaged Fourier transforms of electron images of single particles embedded in ice and the simulated contrast transfer function with or without envelope functions, This interface provides an easy and efficient operation for the determination of defocus value and the evaluation of the extent of Fourier amplitude falloff, This computational procedure is crucial for prescreening image data and performing image correction of contrast transfer function in high-resolution three-dimensional reconstruction of single particles.

Original languageEnglish (US)
Pages (from-to)216-222
Number of pages7
JournalJournal of Structural Biology
Volume116
Issue number1
DOIs
StatePublished - Jan 1996
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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