TY - CHAP
T1 - Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface
AU - Lintao, Ryan C.V.
AU - Richardson, Lauren S.
AU - Chapa, Jenieve
AU - Dalmacio, Leslie Michelle M.
AU - Menon, Ramkumar
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition.
AB - The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition.
KW - Cell culture
KW - Macrophages
KW - Maternal-fetal interface
KW - Natural killer cells
KW - Neutrophils
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U2 - 10.1007/978-1-0716-3746-3_11
DO - 10.1007/978-1-0716-3746-3_11
M3 - Chapter
C2 - 38502448
AN - SCOPUS:85188760804
T3 - Methods in Molecular Biology
SP - 119
EP - 130
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -