Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface

Ryan C.V. Lintao, Lauren S. Richardson, Jenieve Chapa, Leslie Michelle M. Dalmacio, Ramkumar Menon

Research output: Chapter in Book/Report/Conference proceedingChapter


The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cell culture
  • Macrophages
  • Maternal-fetal interface
  • Natural killer cells
  • Neutrophils

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface'. Together they form a unique fingerprint.

Cite this