Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report

Yuanchao Xue; Filipe M. Cerqueira; Heather L. Stevenson; Natalie Williams-Bouyer; Rong Fang

doi:10.1016/j.imj.2025.100180

Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report

Yuanchao Xue
, Filipe M. Cerqueira
, Heather L. Stevenson
, Natalie Williams-Bouyer
, Rong Fang

Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

Pyogenic liver abscess (PLA) is a potentially life-threatening disease. Early diagnosis and appropriate treatment are crucial to ensure high-quality healthcare for patients with PLA. However, this is complicated by their non-specific clinical symptoms. In addition, the etiologic organisms responsible for PLA are frequently culture-negative, thus complicating clinical decision-making. Here, we report a case of PLA caused by Streptococcus intermedius, as identified via DNA metagenomic sequencing of plasma.

Original language	English (US)
Article number	100180
Journal	Infectious Medicine
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/j.imj.2025.100180
State	Published - Jun 2025

Keywords

Next-generation sequencing
Pyogenic liver abscess
Streptococcus anginosus group

ASJC Scopus subject areas

Immunology and Allergy
General Immunology and Microbiology
Microbiology (medical)
Infectious Diseases

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10.1016/j.imj.2025.100180

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title = "Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report",

abstract = "Pyogenic liver abscess (PLA) is a potentially life-threatening disease. Early diagnosis and appropriate treatment are crucial to ensure high-quality healthcare for patients with PLA. However, this is complicated by their non-specific clinical symptoms. In addition, the etiologic organisms responsible for PLA are frequently culture-negative, thus complicating clinical decision-making. Here, we report a case of PLA caused by Streptococcus intermedius, as identified via DNA metagenomic sequencing of plasma.",

keywords = "Next-generation sequencing, Pyogenic liver abscess, Streptococcus anginosus group",

author = "Yuanchao Xue and Cerqueira, \{Filipe M.\} and Stevenson, \{Heather L.\} and Natalie Williams-Bouyer and Rong Fang",

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doi = "10.1016/j.imj.2025.100180",

language = "English (US)",

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T1 - Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing

T2 - A case report

AU - Xue, Yuanchao

AU - Cerqueira, Filipe M.

AU - Stevenson, Heather L.

AU - Williams-Bouyer, Natalie

AU - Fang, Rong

PY - 2025/6

Y1 - 2025/6

N2 - Pyogenic liver abscess (PLA) is a potentially life-threatening disease. Early diagnosis and appropriate treatment are crucial to ensure high-quality healthcare for patients with PLA. However, this is complicated by their non-specific clinical symptoms. In addition, the etiologic organisms responsible for PLA are frequently culture-negative, thus complicating clinical decision-making. Here, we report a case of PLA caused by Streptococcus intermedius, as identified via DNA metagenomic sequencing of plasma.

AB - Pyogenic liver abscess (PLA) is a potentially life-threatening disease. Early diagnosis and appropriate treatment are crucial to ensure high-quality healthcare for patients with PLA. However, this is complicated by their non-specific clinical symptoms. In addition, the etiologic organisms responsible for PLA are frequently culture-negative, thus complicating clinical decision-making. Here, we report a case of PLA caused by Streptococcus intermedius, as identified via DNA metagenomic sequencing of plasma.

KW - Next-generation sequencing

KW - Pyogenic liver abscess

KW - Streptococcus anginosus group

UR - https://www.scopus.com/pages/publications/105005447517

UR - https://www.scopus.com/pages/publications/105005447517#tab=citedBy

U2 - 10.1016/j.imj.2025.100180

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M3 - Article

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JO - Infectious Medicine

JF - Infectious Medicine

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M1 - 100180

ER -

Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report

Abstract

Keywords

ASJC Scopus subject areas

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