Curcumin induces the tolerogenic dendritic cell that promotes differentiation of intestine-protective regulatory T cells

Yingzi Cong, Lanfang Wang, Astrid Konrad, Trenton Schoeb, Charles O. Elson

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The gut is home to a large number of Treg, with both CD4+ CD25+ Treg and bacterial antigen-specific Tr1 cells present in normal mouse intestinal lamina propria. It has been shown recently that intestinal mucosal DC are able to induce Foxp3+ Treg through production of TGF-β plus retinoic acid (RA). However, the factors instructing DC toward this mucosal phenotype are currently unknown. Curcumin has been shown to possess a number of biologic activities including the inhibition of NF-κB signaling. We asked whether curcumin could modulate DC to be tolerogenic whose function could mimic mucosal DC. We report here that curcumin modulated BM-derived DC to express ALDH1a and IL-10. These curcumin-treated DC induced differentiation of naïve CD4+ T cells into Treg resembling Treg in the intestine, including both CD4+CD25+ Foxp3 + Treg and IL-10-producing Tr1 cells. Such Treg induction required IL-10, TGF-β and retinoic acid produced by curcumin-modulated DC. Cell contact as well as IL-10 and TGF-β production were involved in the function of such induced Treg. More importantly, these Treg inhibited antigen-specific T-cell activation in vitro and inhibited colitis due to antigen-specific pathogenic T cells in vivo.

Original languageEnglish (US)
Pages (from-to)3134-3146
Number of pages13
JournalEuropean Journal of Immunology
Volume39
Issue number11
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Curcumin
Regulatory T-Lymphocytes
Dendritic Cells
Intestines
Cell Differentiation
Interleukin-10
Tretinoin
CD27 Antigens
Interleukin-2 Receptor alpha Subunit
Bacterial Antigens
T-Lymphocytes
Colitis
Mucous Membrane
Phenotype
Antigens

Keywords

  • Colitis
  • Curcumin
  • DC
  • Foxp3
  • Treg

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Curcumin induces the tolerogenic dendritic cell that promotes differentiation of intestine-protective regulatory T cells. / Cong, Yingzi; Wang, Lanfang; Konrad, Astrid; Schoeb, Trenton; Elson, Charles O.

In: European Journal of Immunology, Vol. 39, No. 11, 11.2009, p. 3134-3146.

Research output: Contribution to journalArticle

Cong, Yingzi ; Wang, Lanfang ; Konrad, Astrid ; Schoeb, Trenton ; Elson, Charles O. / Curcumin induces the tolerogenic dendritic cell that promotes differentiation of intestine-protective regulatory T cells. In: European Journal of Immunology. 2009 ; Vol. 39, No. 11. pp. 3134-3146.
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