Curcumin promotes autophagic survival of a subset of colon cancer stem cells, which are ablated by DCLK1-siRNA

Carla Kantara, Malaney O'Connell, Shubhashish Sarkar, Stephanie Moya, Robert Ullrich, Pomila Singh

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Curcumin is known to induce apoptosis of cancer cells by different mechanisms, but its effects on cancer stem cells (CSC) have been less investigated. Here, we report that curcumin promotes the survival of DCLK1-positive colon CSCs, potentially confounding application of its anticancer properties. At optimal concentrations, curcumin greatly reduced expression levels of stem cell markers (DCLK1/CD44/ALDHA1/Lgr5/Nanog) in three-dimensional spheroid cultures and tumor xenografts derived from colon cancer cells. However, curcumin unexpectedly induced proliferation and autophagic survival of a subset of DCLK1-positive CSCs. Spheroid cultures were disintegrated by curcumin in vitro but regrew within 30 to 40 days of treatment, suggesting a survival benefit from autophagy, permitting long-term persistence of colorectal cancer. Notably, RNA interference- mediated silencing of DCLK1 triggered apoptotic cell death of colon cancer cells in vitro and in vivo, and abolished colorectal cancer survival in response to curcumin; combination of DCLK1-siRNA and curcumin dramatically reversed CSC phenotype, contributing to attenuation of the growth of spheroid cultures and tumor xenografts. Taken together, our findings confirm a role of DCLK1 in colon CSCs and highlight DCLK1 as a target to enhance antitumor properties of curcumin.

Original languageEnglish (US)
Pages (from-to)2487-2498
Number of pages12
JournalCancer Research
Volume74
Issue number9
DOIs
StatePublished - Jan 5 2014

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Curcumin
Neoplastic Stem Cells
Colonic Neoplasms
Small Interfering RNA
Heterografts
Colorectal Neoplasms
Colon
Neoplasms
Autophagy
RNA Interference
Cell Death
Stem Cells
Apoptosis
Phenotype
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Curcumin promotes autophagic survival of a subset of colon cancer stem cells, which are ablated by DCLK1-siRNA. / Kantara, Carla; O'Connell, Malaney; Sarkar, Shubhashish; Moya, Stephanie; Ullrich, Robert; Singh, Pomila.

In: Cancer Research, Vol. 74, No. 9, 05.01.2014, p. 2487-2498.

Research output: Contribution to journalArticle

Kantara, Carla ; O'Connell, Malaney ; Sarkar, Shubhashish ; Moya, Stephanie ; Ullrich, Robert ; Singh, Pomila. / Curcumin promotes autophagic survival of a subset of colon cancer stem cells, which are ablated by DCLK1-siRNA. In: Cancer Research. 2014 ; Vol. 74, No. 9. pp. 2487-2498.
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