CXCL10 production by human monocytes in response to Leishmania braziliensis Infection

Diego A. Vargas-Inchaustegui, Alison E. Hogg, Gianfranco Tulliano, Alejandro Llanos-Cuentas, Jorge Arevalo, Janice Endsley, Lynn Soong

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Leishmania (subgenus Viannia) braziliensis is the causative agent of mucocutaneous leishmaniasis (ML) in South America, and ML is characterized by excessive T- and B-cell responses to the parasite. We speculate that the unbalanced production of inflammatory mediators in response to L. braziliensis infection contributes to cell recruitment and disease severity. To test this hypothesis, we first examined the response of peripheral blood mononuclear cells (PBMCs) from healthy volunteers to L. braziliensis infection. We observed that while L. braziliensis infection induced the production of chemokine (C-X-C motif) ligand 10 (CXCL10) and interleukin-10 (IL-10) in human PBMCs and macrophages (MΦs), enhanced expression of CXCL10 and its receptor, chemokine CXC receptor (CXCR3), was predominantly detected in CD14+ monocytes. The chemoattractant factors secreted by L. braziliensis-infected cells were highly efficient in recruiting uninfected PBMCs (predominantly CD14+ cells) through Transwell membranes. Serum samples from American tegumentary leishmaniasis (ATL) patients (especially the ML cases) had significantly higher levels of CXCL10, CCL4, and soluble tumor necrosis factor (TNF) receptor II (sTNFRII) than did those of control subjects. Our results suggest that, following L. braziliensis infection, the production of multiple inflammatory mediators by the host may contribute to disease severity by increasing cellular recruitment.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalInfection and Immunity
Volume78
Issue number1
DOIs
StatePublished - Jan 2010

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Leishmania braziliensis
Monocytes
Mucocutaneous Leishmaniasis
Ligands
Infection
Blood Cells
CXCR Receptors
Chemokine CXCL10
Cutaneous Leishmaniasis
South America
Tumor Necrosis Factor Receptors
Chemotactic Factors
Interleukin-10
Healthy Volunteers
Parasites
B-Lymphocytes
Macrophages
Membranes
Serum

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Vargas-Inchaustegui, D. A., Hogg, A. E., Tulliano, G., Llanos-Cuentas, A., Arevalo, J., Endsley, J., & Soong, L. (2010). CXCL10 production by human monocytes in response to Leishmania braziliensis Infection. Infection and Immunity, 78(1), 301-308. https://doi.org/10.1128/IAI.00959-09

CXCL10 production by human monocytes in response to Leishmania braziliensis Infection. / Vargas-Inchaustegui, Diego A.; Hogg, Alison E.; Tulliano, Gianfranco; Llanos-Cuentas, Alejandro; Arevalo, Jorge; Endsley, Janice; Soong, Lynn.

In: Infection and Immunity, Vol. 78, No. 1, 01.2010, p. 301-308.

Research output: Contribution to journalArticle

Vargas-Inchaustegui, DA, Hogg, AE, Tulliano, G, Llanos-Cuentas, A, Arevalo, J, Endsley, J & Soong, L 2010, 'CXCL10 production by human monocytes in response to Leishmania braziliensis Infection', Infection and Immunity, vol. 78, no. 1, pp. 301-308. https://doi.org/10.1128/IAI.00959-09
Vargas-Inchaustegui DA, Hogg AE, Tulliano G, Llanos-Cuentas A, Arevalo J, Endsley J et al. CXCL10 production by human monocytes in response to Leishmania braziliensis Infection. Infection and Immunity. 2010 Jan;78(1):301-308. https://doi.org/10.1128/IAI.00959-09
Vargas-Inchaustegui, Diego A. ; Hogg, Alison E. ; Tulliano, Gianfranco ; Llanos-Cuentas, Alejandro ; Arevalo, Jorge ; Endsley, Janice ; Soong, Lynn. / CXCL10 production by human monocytes in response to Leishmania braziliensis Infection. In: Infection and Immunity. 2010 ; Vol. 78, No. 1. pp. 301-308.
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