Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise

Kevin A. Murach, R. Grace Walton, Christopher Fry, Sami L. Michaelis, Jason S. Groshong, Brian S. Finlin, Philip A. Kern, Charlotte A. Peterson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This investigation evaluated whether moderate-intensity cycle ergometer training affects satellite cell and molecular responses to acute maximal concentric/eccentric resistance exercise in middle-aged women. Baseline and 72 h postresistance exercise vastus lateralis biopsies were obtained from seven healthy middle-aged women (56 ± 5 years, BMI 26 ± 1, VO2max 27 ± 4) before and after 12 weeks of cycle training. Myosin heavy chain (MyHC) I- and II-associated satellite cell density and cross-sectional area was determined via immunohistochemistry. Expression of 93 genes representative of the muscle-remodeling environment was also measured via NanoString. Overall fiber size increased ~20% with cycle training (P = 0.052). MyHC I satellite cell density increased 29% in response to acute resistance exercise before endurance training and 50% with endurance training (P < 0.05). Following endurance training, MyHC I satellite cell density decreased by 13% in response to acute resistance exercise (acute resistance × training interaction, P < 0.05). Genes with an interaction effect tracked with satellite cell behavior, increasing in the untrained state and decreasing in the endurance trained state in response to resistance exercise. Similar satellite cell and gene expression response patterns indicate coordinated regulation of the muscle environment to promote adaptation. Moderate-intensity endurance cycle training modulates the response to acute resistance exercise, potentially conditioning the muscle for more intense concentric/eccentric activity. These results suggest that cycle training is an effective endurance exercise modality for promoting growth in middle-aged women, who are susceptible to muscle mass loss with progressing age.

Original languageEnglish (US)
Article numbere12973
JournalPhysiological Reports
Volume4
Issue number18
DOIs
StatePublished - Sep 1 2016

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Exercise
Myosin Heavy Chains
Muscles
Cell Count
Gene Expression
Quadriceps Muscle
Immunohistochemistry
Biopsy
Growth
Genes

Keywords

  • Acute exercise
  • aerobic training
  • cytokines
  • fiber type-specific

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Murach, K. A., Walton, R. G., Fry, C., Michaelis, S. L., Groshong, J. S., Finlin, B. S., ... Peterson, C. A. (2016). Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise. Physiological Reports, 4(18), [e12973]. https://doi.org/10.14814/phy2.12973

Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise. / Murach, Kevin A.; Walton, R. Grace; Fry, Christopher; Michaelis, Sami L.; Groshong, Jason S.; Finlin, Brian S.; Kern, Philip A.; Peterson, Charlotte A.

In: Physiological Reports, Vol. 4, No. 18, e12973, 01.09.2016.

Research output: Contribution to journalArticle

Murach, KA, Walton, RG, Fry, C, Michaelis, SL, Groshong, JS, Finlin, BS, Kern, PA & Peterson, CA 2016, 'Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise', Physiological Reports, vol. 4, no. 18, e12973. https://doi.org/10.14814/phy2.12973
Murach, Kevin A. ; Walton, R. Grace ; Fry, Christopher ; Michaelis, Sami L. ; Groshong, Jason S. ; Finlin, Brian S. ; Kern, Philip A. ; Peterson, Charlotte A. / Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise. In: Physiological Reports. 2016 ; Vol. 4, No. 18.
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