Cyclic stretching of soft substrates induces spreading and growth

Yidan Cui, Feroz M. Hameed, Bo Yang, Kyunghee Lee, Catherine Qiurong Pan, Sungsu Park, Michael Sheetz

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

In the body, soft tissues often undergo cycles of stretching and relaxation that may affect cell behaviour without changing matrix rigidity. To determine whether transient forces can substitute for a rigid matrix, we stretched soft pillar arrays. Surprisingly, 1-5% cyclic stretching over a frequency range of 0.01-10 Hz caused spreading and stress fibre formation (optimum 0.1 Hz) that persisted after 4 h of stretching. Similarly, stretching increased cell growth rates on soft pillars comparative to rigid substrates. Of possible factors linked to fibroblast growth, MRTF-A (myocardin-related transcription factor-A) moved to the nucleus in 2 h of cyclic stretching and reversed on cessation; but YAP (Yes-associated protein) moved much later. Knockdown of either MRTF-A or YAP blocked stretch-dependent growth. Thus, we suggest that the repeated pulling from a soft matrix can substitute for a stiff matrix in stimulating spreading, stress fibre formation and growth.

Original languageEnglish (US)
Article number6333
JournalNature communications
Volume6
DOIs
StatePublished - Feb 23 2015
Externally publishedYes

Fingerprint

Stretching
Stress Fibers
Substrates
Growth
matrices
Transcription Factors
substitutes
proteins
fibers
Fibers
pulling
fibroblasts
Cell growth
Fibroblasts
Proteins
rigidity
Rigidity
frequency ranges
Tissue
cycles

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Cyclic stretching of soft substrates induces spreading and growth. / Cui, Yidan; Hameed, Feroz M.; Yang, Bo; Lee, Kyunghee; Pan, Catherine Qiurong; Park, Sungsu; Sheetz, Michael.

In: Nature communications, Vol. 6, 6333, 23.02.2015.

Research output: Contribution to journalArticle

Cui Y, Hameed FM, Yang B, Lee K, Pan CQ, Park S et al. Cyclic stretching of soft substrates induces spreading and growth. Nature communications. 2015 Feb 23;6. 6333. https://doi.org/10.1038/ncomms7333
Cui, Yidan ; Hameed, Feroz M. ; Yang, Bo ; Lee, Kyunghee ; Pan, Catherine Qiurong ; Park, Sungsu ; Sheetz, Michael. / Cyclic stretching of soft substrates induces spreading and growth. In: Nature communications. 2015 ; Vol. 6.
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