Cyclosporin A inhibits the replication of diverse coronaviruses

Adriaan H. de Wilde, Jessika C. Zevenhoven-Dobbe, Yvonne van der Meer, Volker Thiel, Krishna Narayanan, Shinji Makino, Eric J. Snijder, Martijn J. van Hemert

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

Original languageEnglish (US)
Pages (from-to)2542-2548
Number of pages7
JournalJournal of General Virology
Volume92
Issue number11
DOIs
StatePublished - Nov 2011

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Severe Acute Respiratory Syndrome
Coronavirus
Cyclosporine
Human Coronavirus 229E
Cyclophilin A
Murine hepatitis virus
Cyclophilins
Reporter Genes
Small Interfering RNA
Cell Culture Techniques
RNA
Gene Expression
Infection
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

de Wilde, A. H., Zevenhoven-Dobbe, J. C., van der Meer, Y., Thiel, V., Narayanan, K., Makino, S., ... van Hemert, M. J. (2011). Cyclosporin A inhibits the replication of diverse coronaviruses. Journal of General Virology, 92(11), 2542-2548. https://doi.org/10.1099/vir.0.034983-0

Cyclosporin A inhibits the replication of diverse coronaviruses. / de Wilde, Adriaan H.; Zevenhoven-Dobbe, Jessika C.; van der Meer, Yvonne; Thiel, Volker; Narayanan, Krishna; Makino, Shinji; Snijder, Eric J.; van Hemert, Martijn J.

In: Journal of General Virology, Vol. 92, No. 11, 11.2011, p. 2542-2548.

Research output: Contribution to journalArticle

de Wilde, AH, Zevenhoven-Dobbe, JC, van der Meer, Y, Thiel, V, Narayanan, K, Makino, S, Snijder, EJ & van Hemert, MJ 2011, 'Cyclosporin A inhibits the replication of diverse coronaviruses', Journal of General Virology, vol. 92, no. 11, pp. 2542-2548. https://doi.org/10.1099/vir.0.034983-0
de Wilde AH, Zevenhoven-Dobbe JC, van der Meer Y, Thiel V, Narayanan K, Makino S et al. Cyclosporin A inhibits the replication of diverse coronaviruses. Journal of General Virology. 2011 Nov;92(11):2542-2548. https://doi.org/10.1099/vir.0.034983-0
de Wilde, Adriaan H. ; Zevenhoven-Dobbe, Jessika C. ; van der Meer, Yvonne ; Thiel, Volker ; Narayanan, Krishna ; Makino, Shinji ; Snijder, Eric J. ; van Hemert, Martijn J. / Cyclosporin A inhibits the replication of diverse coronaviruses. In: Journal of General Virology. 2011 ; Vol. 92, No. 11. pp. 2542-2548.
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