Cyclosporin A inhibits the replication of diverse coronaviruses

Adriaan H. de Wilde, Jessika C. Zevenhoven-Dobbe, Yvonne van der Meer, Volker Thiel, Krishna Narayanan, Shinji Makino, Eric J. Snijder, Martijn J. van Hemert

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

Original languageEnglish (US)
Pages (from-to)2542-2548
Number of pages7
JournalJournal of General Virology
Volume92
Issue number11
DOIs
StatePublished - Nov 2011

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Cyclosporin A inhibits the replication of diverse coronaviruses'. Together they form a unique fingerprint.

Cite this