Cyclosporin A inhibits the replication of diverse coronaviruses

Adriaan H. de Wilde, Jessika C. Zevenhoven-Dobbe, Yvonne van der Meer, Volker Thiel, Krishna Narayanan, Shinji Makino, Eric J. Snijder, Martijn J. van Hemert

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

Original languageEnglish (US)
Pages (from-to)2542-2548
Number of pages7
JournalJournal of General Virology
Volume92
Issue number11
DOIs
StatePublished - Nov 1 2011

ASJC Scopus subject areas

  • Virology

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    de Wilde, A. H., Zevenhoven-Dobbe, J. C., van der Meer, Y., Thiel, V., Narayanan, K., Makino, S., Snijder, E. J., & van Hemert, M. J. (2011). Cyclosporin A inhibits the replication of diverse coronaviruses. Journal of General Virology, 92(11), 2542-2548. https://doi.org/10.1099/vir.0.034983-0