Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein

Min Qing, Feng Yang, Bo Zhang, Gang Zou, John M. Robida, Zhiming Yuan, Hengli Tang, Pei-Yong Shi

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Although flaviviruses cause significant human diseases, no effective therapy is currently available. Host factors essential for viral replication are potential targets for antiviral development. Here we report that cyclophilins (CyPs), a family of cellular peptidyl-prolyl isomerases (PPIases), play a role in flavivirus replication. Huh-7.5 cells with knockdown of different isoforms of CyP were less efficient than parental cells in supporting flavivirus replication, including West Nile virus (WNV), dengue virus, and yellow fever virus. The low viral replication in CyP A (CyPA) knockdown cells could be rescued by trans supplying of a wild-type CyPA but not by trans supplying of a mutant CyPA (defective in the PPIase activity), indicating that the isomerase activity of CyPA is critical for viral replication. Immunoprecipitation and biochemical pulldown analyses showed that CyPA interacts with WNV genomic RNA and viral NS5 protein in the replication complex. Furthermore, antiviral experiments demonstrated that cyclosporine (Cs; an 11-amino-acid cyclic peptide known to block the PPIase activity of CyPA) inhibits flavivirus replication in cell culture at nontoxic concentrations. Time-of-addition and transient replicon results indicated that Cs inhibits flavivirus at the step of viral RNA synthesis. Biochemical analysis showed that Cs directly blocks the interaction between CyPA and WNV NS5 protein. Our results suggest that host CyPA is a component of flavivirus replication complex and could be targeted for potential antiviral development.

Original languageEnglish (US)
Pages (from-to)3226-3235
Number of pages10
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number8
DOIs
StatePublished - Aug 2009
Externally publishedYes

Fingerprint

Cyclophilins
Flavivirus
Viral Proteins
Cyclosporine
Peptidylprolyl Isomerase
Antiviral Agents
West Nile virus
Yellow fever virus
Isomerases
Cyclic Peptides
Replicon
Dengue Virus
Dengue
Viral RNA
Immunoprecipitation
Protein Isoforms
Cell Culture Techniques
RNA
Amino Acids

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein. / Qing, Min; Yang, Feng; Zhang, Bo; Zou, Gang; Robida, John M.; Yuan, Zhiming; Tang, Hengli; Shi, Pei-Yong.

In: Antimicrobial Agents and Chemotherapy, Vol. 53, No. 8, 08.2009, p. 3226-3235.

Research output: Contribution to journalArticle

Qing, Min ; Yang, Feng ; Zhang, Bo ; Zou, Gang ; Robida, John M. ; Yuan, Zhiming ; Tang, Hengli ; Shi, Pei-Yong. / Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein. In: Antimicrobial Agents and Chemotherapy. 2009 ; Vol. 53, No. 8. pp. 3226-3235.
@article{0a6b1945aa714603b5a9d93b09a70aa6,
title = "Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein",
abstract = "Although flaviviruses cause significant human diseases, no effective therapy is currently available. Host factors essential for viral replication are potential targets for antiviral development. Here we report that cyclophilins (CyPs), a family of cellular peptidyl-prolyl isomerases (PPIases), play a role in flavivirus replication. Huh-7.5 cells with knockdown of different isoforms of CyP were less efficient than parental cells in supporting flavivirus replication, including West Nile virus (WNV), dengue virus, and yellow fever virus. The low viral replication in CyP A (CyPA) knockdown cells could be rescued by trans supplying of a wild-type CyPA but not by trans supplying of a mutant CyPA (defective in the PPIase activity), indicating that the isomerase activity of CyPA is critical for viral replication. Immunoprecipitation and biochemical pulldown analyses showed that CyPA interacts with WNV genomic RNA and viral NS5 protein in the replication complex. Furthermore, antiviral experiments demonstrated that cyclosporine (Cs; an 11-amino-acid cyclic peptide known to block the PPIase activity of CyPA) inhibits flavivirus replication in cell culture at nontoxic concentrations. Time-of-addition and transient replicon results indicated that Cs inhibits flavivirus at the step of viral RNA synthesis. Biochemical analysis showed that Cs directly blocks the interaction between CyPA and WNV NS5 protein. Our results suggest that host CyPA is a component of flavivirus replication complex and could be targeted for potential antiviral development.",
author = "Min Qing and Feng Yang and Bo Zhang and Gang Zou and Robida, {John M.} and Zhiming Yuan and Hengli Tang and Pei-Yong Shi",
year = "2009",
month = "8",
doi = "10.1128/AAC.00189-09",
language = "English (US)",
volume = "53",
pages = "3226--3235",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein

AU - Qing, Min

AU - Yang, Feng

AU - Zhang, Bo

AU - Zou, Gang

AU - Robida, John M.

AU - Yuan, Zhiming

AU - Tang, Hengli

AU - Shi, Pei-Yong

PY - 2009/8

Y1 - 2009/8

N2 - Although flaviviruses cause significant human diseases, no effective therapy is currently available. Host factors essential for viral replication are potential targets for antiviral development. Here we report that cyclophilins (CyPs), a family of cellular peptidyl-prolyl isomerases (PPIases), play a role in flavivirus replication. Huh-7.5 cells with knockdown of different isoforms of CyP were less efficient than parental cells in supporting flavivirus replication, including West Nile virus (WNV), dengue virus, and yellow fever virus. The low viral replication in CyP A (CyPA) knockdown cells could be rescued by trans supplying of a wild-type CyPA but not by trans supplying of a mutant CyPA (defective in the PPIase activity), indicating that the isomerase activity of CyPA is critical for viral replication. Immunoprecipitation and biochemical pulldown analyses showed that CyPA interacts with WNV genomic RNA and viral NS5 protein in the replication complex. Furthermore, antiviral experiments demonstrated that cyclosporine (Cs; an 11-amino-acid cyclic peptide known to block the PPIase activity of CyPA) inhibits flavivirus replication in cell culture at nontoxic concentrations. Time-of-addition and transient replicon results indicated that Cs inhibits flavivirus at the step of viral RNA synthesis. Biochemical analysis showed that Cs directly blocks the interaction between CyPA and WNV NS5 protein. Our results suggest that host CyPA is a component of flavivirus replication complex and could be targeted for potential antiviral development.

AB - Although flaviviruses cause significant human diseases, no effective therapy is currently available. Host factors essential for viral replication are potential targets for antiviral development. Here we report that cyclophilins (CyPs), a family of cellular peptidyl-prolyl isomerases (PPIases), play a role in flavivirus replication. Huh-7.5 cells with knockdown of different isoforms of CyP were less efficient than parental cells in supporting flavivirus replication, including West Nile virus (WNV), dengue virus, and yellow fever virus. The low viral replication in CyP A (CyPA) knockdown cells could be rescued by trans supplying of a wild-type CyPA but not by trans supplying of a mutant CyPA (defective in the PPIase activity), indicating that the isomerase activity of CyPA is critical for viral replication. Immunoprecipitation and biochemical pulldown analyses showed that CyPA interacts with WNV genomic RNA and viral NS5 protein in the replication complex. Furthermore, antiviral experiments demonstrated that cyclosporine (Cs; an 11-amino-acid cyclic peptide known to block the PPIase activity of CyPA) inhibits flavivirus replication in cell culture at nontoxic concentrations. Time-of-addition and transient replicon results indicated that Cs inhibits flavivirus at the step of viral RNA synthesis. Biochemical analysis showed that Cs directly blocks the interaction between CyPA and WNV NS5 protein. Our results suggest that host CyPA is a component of flavivirus replication complex and could be targeted for potential antiviral development.

UR - http://www.scopus.com/inward/record.url?scp=67749119973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67749119973&partnerID=8YFLogxK

U2 - 10.1128/AAC.00189-09

DO - 10.1128/AAC.00189-09

M3 - Article

VL - 53

SP - 3226

EP - 3235

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 8

ER -