Cystic fibrosis: molecular weight estimation of the ciliary inhibitor

D. R. Barnett, A. Kurosky, B. H. Bowman, H. T. Hutchison, I. Schmoyer, S. D. Carson

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The molecular weight of the ciliary inhibitor fractionated from media of cultured fibroblasts derived from cystic fibrosis patients was estimated by sucrose density gradient ultracentrifugation, membrane filtration and gel filtration. Ultracentrifugation analysis indicated a molecular weight closer to the α 1 chain of haptoglobin (9,100) than to immunoglobulin G (150,000). Molecular filtration on Amicon membranes indicated a molecular weight range of 1,000 to 10,000. Gel filtration on Sephadex G 50F indicated a molecular weight of 4,500 to 10,000. These results characterize the ciliary inhibitor from fibroblast media as a small molecular weight protein.

    Original languageEnglish (US)
    Pages (from-to)703-708
    Number of pages6
    JournalTexas Reports on Biology and Medicine
    Volume31
    Issue number4
    StatePublished - 1973

    Fingerprint

    Cystic Fibrosis
    Molecular Weight
    Ultracentrifugation
    Gel Chromatography
    Fibroblasts
    Haptoglobins
    Membranes
    Sucrose
    Immunoglobulin G
    Proteins

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Barnett, D. R., Kurosky, A., Bowman, B. H., Hutchison, H. T., Schmoyer, I., & Carson, S. D. (1973). Cystic fibrosis: molecular weight estimation of the ciliary inhibitor. Texas Reports on Biology and Medicine, 31(4), 703-708.

    Cystic fibrosis : molecular weight estimation of the ciliary inhibitor. / Barnett, D. R.; Kurosky, A.; Bowman, B. H.; Hutchison, H. T.; Schmoyer, I.; Carson, S. D.

    In: Texas Reports on Biology and Medicine, Vol. 31, No. 4, 1973, p. 703-708.

    Research output: Contribution to journalArticle

    Barnett, DR, Kurosky, A, Bowman, BH, Hutchison, HT, Schmoyer, I & Carson, SD 1973, 'Cystic fibrosis: molecular weight estimation of the ciliary inhibitor', Texas Reports on Biology and Medicine, vol. 31, no. 4, pp. 703-708.
    Barnett DR, Kurosky A, Bowman BH, Hutchison HT, Schmoyer I, Carson SD. Cystic fibrosis: molecular weight estimation of the ciliary inhibitor. Texas Reports on Biology and Medicine. 1973;31(4):703-708.
    Barnett, D. R. ; Kurosky, A. ; Bowman, B. H. ; Hutchison, H. T. ; Schmoyer, I. ; Carson, S. D. / Cystic fibrosis : molecular weight estimation of the ciliary inhibitor. In: Texas Reports on Biology and Medicine. 1973 ; Vol. 31, No. 4. pp. 703-708.
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