Cystic fibrosis: Synthesis of ciliary inhibitor by amniotic cells

Barbara H. Bowman, Lillian H. Lockhart, Victoria L. Herzberg, Don R. Barnett, Donald Armstrong, John Kramer

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The presence of a ciliary inhibitor in media of cultured amniotic cells obtained from a fetus heterozygous for cystic fibrosis has been observed by the oyster gill cilia assay. The chromatographic fraction containing the inhibitor corresponded to eluted fractions chromatographed from cystic fibrosis fibroblast media and serum. An analogous chromatographic fraction from media of cultured amniotic cells from two proportedly normal fetuses did not inhibit cilia. The chromatographic fraction from media of cultured amniotic cells of a fetus at high risk for cystic fibrosis did not inhibit ciliary activity. Serum was collected from this baby seven weeks after birth and also did not inhibit ciliary action, indicating a homozygous normal genotype. These observations may lead to the development of an antenatal test for cystic fibrosis.

Original languageEnglish (US)
Pages (from-to)461-463
Number of pages3
JournalClinical Genetics
Volume4
Issue number6
DOIs
StatePublished - Nov 1973

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Bowman, B. H., Lockhart, L. H., Herzberg, V. L., Barnett, D. R., Armstrong, D., & Kramer, J. (1973). Cystic fibrosis: Synthesis of ciliary inhibitor by amniotic cells. Clinical Genetics, 4(6), 461-463. https://doi.org/10.1111/j.1399-0004.1973.tb01933.x