Cytochrome c-mediated apoptosis in cells lacking mitochondrial DNA. Signaling pathway involving release and caspase 3 activation is conserved

Shunai Jiang, Jiyang Cai, Douglas C. Wallace, Dean P. Jones

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Mitochondria serve as a pivotal component of the apoptotic cell death machinery. However, cells that lack mitochondrial DNA (ρ0 cells) retain apparently normal apoptotic signaling. In the present study, we examined mitochondrial mechanisms of apoptosis in ρ0 osteosarcoma cells treated with staurosporine. Immunohistochemistry revealed that ρ0 cells maintained a normal cytochrome c distribution in mitochondria even though these cells were deficient in respiration. Upon staurosporine treatment, cytochrome c was released concomitantly with activation of caspase 3 and loss of mitochondrial membrane potential (Aψ(m)). After mitochondrial loss of cytochrome c, ρ0 cells underwent little change in glutathione (GSH) redox potential whereas a dramatic oxidation in GSH/glutathione disulfide (GSSG) pool occurred in parental ρ+ cells. These results show that mitochondrial signaling of apoptosis via cytochrome c release was preserved in cells lacking mtDNA. However, intracellular oxidation that normally accompanies apoptosis was lost, indicating that the mitochondrial respiratory chain provides the major source of redox signaling in apoptosis.

Original languageEnglish (US)
Pages (from-to)29905-29911
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number42
DOIs
StatePublished - Oct 15 1999
Externally publishedYes

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Cytochromes c
Mitochondrial DNA
Caspase 3
Chemical activation
Apoptosis
Mitochondria
Staurosporine
Glutathione Disulfide
Oxidation
Cell death
Oxidation-Reduction
Machinery
Glutathione
Membranes
Mitochondrial Membrane Potential
Osteosarcoma
Electron Transport
Respiration
Cell Death
Immunohistochemistry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cytochrome c-mediated apoptosis in cells lacking mitochondrial DNA. Signaling pathway involving release and caspase 3 activation is conserved. / Jiang, Shunai; Cai, Jiyang; Wallace, Douglas C.; Jones, Dean P.

In: Journal of Biological Chemistry, Vol. 274, No. 42, 15.10.1999, p. 29905-29911.

Research output: Contribution to journalArticle

Jiang, Shunai ; Cai, Jiyang ; Wallace, Douglas C. ; Jones, Dean P. / Cytochrome c-mediated apoptosis in cells lacking mitochondrial DNA. Signaling pathway involving release and caspase 3 activation is conserved. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 42. pp. 29905-29911.
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