Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures

Julianne Meyne, Lillian H. Lockhart

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.

Original languageEnglish (US)
Pages (from-to)87-97
Number of pages11
JournalMutation Research/Genetic Toxicology
Volume58
Issue number1
DOIs
StatePublished - Sep 1978

ASJC Scopus subject areas

  • Toxicology
  • Genetics

Fingerprint Dive into the research topics of 'Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures'. Together they form a unique fingerprint.

  • Cite this