Abstract
Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 87-97 |
| Number of pages | 11 |
| Journal | Mutation Research/Genetic Toxicology |
| Volume | 58 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1978 |
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ASJC Scopus subject areas
- Genetics
- Toxicology
- Medicine(all)
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Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures. / Meyne, Julianne; Lockhart, Lillian.
In: Mutation Research/Genetic Toxicology, Vol. 58, No. 1, 1978, p. 87-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures
AU - Meyne, Julianne
AU - Lockhart, Lillian
PY - 1978
Y1 - 1978
N2 - Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.
AB - Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0018077997&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018077997&partnerID=8YFLogxK
U2 - 10.1016/0165-1218(78)90098-8
DO - 10.1016/0165-1218(78)90098-8
M3 - Article
C2 - 714079
AN - SCOPUS:0018077997
VL - 58
SP - 87
EP - 97
JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
SN - 0165-1218
IS - 1
ER -