Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures

Julianne Meyne, Lillian Lockhart

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Human lymphocytes were treated with the antitumor agent cis-platinum(II)-diamminedichloride (PDD) during either the last 24 h or 48 h of incubation. A dose-dependent effect was observed for both inhibition of mitotic activity and increased frequency of metaphases with chromosomal aberrations. The aberrations observed consisted primarily of chromatid breaks. Statistical analysis of 3244 PDD-induced breakpoints demonstrated a significantly non-random distribution of breakpoints between chromosomes. The pattern of distribution varied with the type of aberration. Only chromosome number 9 had a significant increase of breakpoints for each type of aberration analyzed. The breakpoints were located predominantly in lightly staining G-bands. Certain individual bands had relatively high frequencies of breakpoints, indicating a specific interaction occurs between PDD and the DNA of human lymphocytes in vitro.

Original languageEnglish (US)
Pages (from-to)87-97
Number of pages11
JournalMutation Research/Genetic Toxicology
Volume58
Issue number1
DOIs
StatePublished - 1978

Fingerprint

Lymphocytes
Aberrations
Cell culture
Cytogenetics
Cisplatin
Chromosome Breakpoints
Chromosomes, Human, Pair 9
Chromatids
Metaphase
Chromosomes
Chromosome Aberrations
Antineoplastic Agents
Staining and Labeling
DNA
Statistical methods
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

Cytogenetic effects of cis-platinum(II)diamminedichloride on human lymphocyte cultures. / Meyne, Julianne; Lockhart, Lillian.

In: Mutation Research/Genetic Toxicology, Vol. 58, No. 1, 1978, p. 87-97.

Research output: Contribution to journalArticle

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