Cytokine expression profile over time in burned mice

Celeste Finnerty, Rene Przkora, David Herndon, Marc G. Jeschke

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here, we present the temporal serum cytokine expression profiles in burned mice in comparison to sham mice and human burn patients. Male C57BL/6 mice were randomized to control (n = 47) or subjected to a 35% TBSA scald burn (n = 89). Mice were sacrificed 3, 6, 9, 12, 24, and 48 h and 7, 10, and 14 days post-burn; cytokines were measured by multi-plex array. Following the burn injury, IL-6, IL-1β, KC, G-CSF, TNF, IL-17, MIP-1α, RANTES, and GM-CSF were increased, p < 0.05. IL-2, IL-3, and IL-5 were decreased, p < 0.05. IL-10, IFN-γ, and IL-12p70 were expressed in a biphasic manner, p < 0.05. This temporal cytokine expression pattern elucidates the pathogenesis of the inflammatory response in burned mice. Expression of 11 cytokines were similar in mice and children, returning to lowest levels by post-burn day 14, confirming the utility of the burned mouse model for development of therapeutic interventions to attenuate the post-burn inflammatory response.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalCytokine
Volume45
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Cytokines
Burns
Chemokine CCL5
Interleukin-17
Interleukin-3
Interleukin-5
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Interleukin-10
Interleukin-2
Interleukin-6
Inbred C57BL Mouse
Sepsis
Wounds and Injuries
Infection
Serum
Therapeutics

Keywords

  • Burn
  • Cytokine
  • Human
  • Inflammation
  • Mice

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Cytokine expression profile over time in burned mice. / Finnerty, Celeste; Przkora, Rene; Herndon, David; Jeschke, Marc G.

In: Cytokine, Vol. 45, No. 1, 01.2009, p. 20-25.

Research output: Contribution to journalArticle

Finnerty, C, Przkora, R, Herndon, D & Jeschke, MG 2009, 'Cytokine expression profile over time in burned mice', Cytokine, vol. 45, no. 1, pp. 20-25. https://doi.org/10.1016/j.cyto.2008.10.005
Finnerty, Celeste ; Przkora, Rene ; Herndon, David ; Jeschke, Marc G. / Cytokine expression profile over time in burned mice. In: Cytokine. 2009 ; Vol. 45, No. 1. pp. 20-25.
@article{de1771facd9e4fe294e6fcfab226b148,
title = "Cytokine expression profile over time in burned mice",
abstract = "The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here, we present the temporal serum cytokine expression profiles in burned mice in comparison to sham mice and human burn patients. Male C57BL/6 mice were randomized to control (n = 47) or subjected to a 35{\%} TBSA scald burn (n = 89). Mice were sacrificed 3, 6, 9, 12, 24, and 48 h and 7, 10, and 14 days post-burn; cytokines were measured by multi-plex array. Following the burn injury, IL-6, IL-1β, KC, G-CSF, TNF, IL-17, MIP-1α, RANTES, and GM-CSF were increased, p < 0.05. IL-2, IL-3, and IL-5 were decreased, p < 0.05. IL-10, IFN-γ, and IL-12p70 were expressed in a biphasic manner, p < 0.05. This temporal cytokine expression pattern elucidates the pathogenesis of the inflammatory response in burned mice. Expression of 11 cytokines were similar in mice and children, returning to lowest levels by post-burn day 14, confirming the utility of the burned mouse model for development of therapeutic interventions to attenuate the post-burn inflammatory response.",
keywords = "Burn, Cytokine, Human, Inflammation, Mice",
author = "Celeste Finnerty and Rene Przkora and David Herndon and Jeschke, {Marc G.}",
year = "2009",
month = "1",
doi = "10.1016/j.cyto.2008.10.005",
language = "English (US)",
volume = "45",
pages = "20--25",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cytokine expression profile over time in burned mice

AU - Finnerty, Celeste

AU - Przkora, Rene

AU - Herndon, David

AU - Jeschke, Marc G.

PY - 2009/1

Y1 - 2009/1

N2 - The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here, we present the temporal serum cytokine expression profiles in burned mice in comparison to sham mice and human burn patients. Male C57BL/6 mice were randomized to control (n = 47) or subjected to a 35% TBSA scald burn (n = 89). Mice were sacrificed 3, 6, 9, 12, 24, and 48 h and 7, 10, and 14 days post-burn; cytokines were measured by multi-plex array. Following the burn injury, IL-6, IL-1β, KC, G-CSF, TNF, IL-17, MIP-1α, RANTES, and GM-CSF were increased, p < 0.05. IL-2, IL-3, and IL-5 were decreased, p < 0.05. IL-10, IFN-γ, and IL-12p70 were expressed in a biphasic manner, p < 0.05. This temporal cytokine expression pattern elucidates the pathogenesis of the inflammatory response in burned mice. Expression of 11 cytokines were similar in mice and children, returning to lowest levels by post-burn day 14, confirming the utility of the burned mouse model for development of therapeutic interventions to attenuate the post-burn inflammatory response.

AB - The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here, we present the temporal serum cytokine expression profiles in burned mice in comparison to sham mice and human burn patients. Male C57BL/6 mice were randomized to control (n = 47) or subjected to a 35% TBSA scald burn (n = 89). Mice were sacrificed 3, 6, 9, 12, 24, and 48 h and 7, 10, and 14 days post-burn; cytokines were measured by multi-plex array. Following the burn injury, IL-6, IL-1β, KC, G-CSF, TNF, IL-17, MIP-1α, RANTES, and GM-CSF were increased, p < 0.05. IL-2, IL-3, and IL-5 were decreased, p < 0.05. IL-10, IFN-γ, and IL-12p70 were expressed in a biphasic manner, p < 0.05. This temporal cytokine expression pattern elucidates the pathogenesis of the inflammatory response in burned mice. Expression of 11 cytokines were similar in mice and children, returning to lowest levels by post-burn day 14, confirming the utility of the burned mouse model for development of therapeutic interventions to attenuate the post-burn inflammatory response.

KW - Burn

KW - Cytokine

KW - Human

KW - Inflammation

KW - Mice

UR - http://www.scopus.com/inward/record.url?scp=57749092786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57749092786&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2008.10.005

DO - 10.1016/j.cyto.2008.10.005

M3 - Article

VL - 45

SP - 20

EP - 25

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -