Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia: Cricetidae) infected by Leishmania (Leishmania) mexicana

Elsy Nalleli Loria-Cervera, Erika Ivett Sosa-Bibiano, Nicole Raymonde Van Wynsberghe, Omar Abdul Saldarriaga, Peter Melby, Fernando Jose Andrade-Narvaez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peromyscus yucatanicus, the main reservoir of Leishmania (Leishmania) mexicana in the Yucatan peninsula of Mexico, reproduces clinical and histological pictures of LCL in human as well as subclinical infection. Thus, we used this rodent as a novel experimental model. In this work, we analyzed cytokine mRNA expression in P. yucatanicus infected with L. (L.) mexicana. Animals were inoculated with either 2.5 × 106 or 1 × 102 promastigotes and cytokine expressions were analyzed by real-time RT-PCR in skin at 4 and 12 weeks post-infection (wpi). Independently of the parasite inoculum none of the infected rodents had clinical signs of LCL at 4 wpi and all expressed high IFN-γ mRNA. All P. yucatanicus inoculated with 2.5 × 106 promastigotes developed signs of LCL at 12 wpi while the mice inoculated with 1 × 102 remained subclinical. At that time, both IFN-γ and IL-10 were expressed in P. yucatanicus with clinical and subclinical infections. Expressions of TNF-α and IL-4 were significantly higher in clinical animals (2.5 × 106) compared with subclinical ones (1 × 102). High TGF-β expression was observed in P. yucatanicus with clinical signs when compared with healthy animals. Results suggested that the clinical course of L. (L.) mexicana infection in P. yucatanicus was associated with a specific local pattern of cytokine production at 12 wpi.

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalCytokine
Volume83
DOIs
StatePublished - Jul 1 2016

Fingerprint

Leishmania mexicana
Peromyscus
Arvicolinae
Rodentia
Animals
Cytokines
Messenger RNA
Infection
Asymptomatic Infections
Interleukin-4
Interleukin-10
Skin
Mexico
Real-Time Polymerase Chain Reaction
Parasites
Theoretical Models

Keywords

  • Clinical infection
  • Leishmania (L.) mexicana
  • Peromyscus yucatanicus
  • Subclinical infection
  • Th1/Th2 cytokines

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Loria-Cervera, E. N., Sosa-Bibiano, E. I., Van Wynsberghe, N. R., Saldarriaga, O. A., Melby, P., & Andrade-Narvaez, F. J. (2016). Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia: Cricetidae) infected by Leishmania (Leishmania) mexicana. Cytokine, 83, 176-181. https://doi.org/10.1016/j.cyto.2016.04.016

Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia : Cricetidae) infected by Leishmania (Leishmania) mexicana. / Loria-Cervera, Elsy Nalleli; Sosa-Bibiano, Erika Ivett; Van Wynsberghe, Nicole Raymonde; Saldarriaga, Omar Abdul; Melby, Peter; Andrade-Narvaez, Fernando Jose.

In: Cytokine, Vol. 83, 01.07.2016, p. 176-181.

Research output: Contribution to journalArticle

Loria-Cervera, EN, Sosa-Bibiano, EI, Van Wynsberghe, NR, Saldarriaga, OA, Melby, P & Andrade-Narvaez, FJ 2016, 'Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia: Cricetidae) infected by Leishmania (Leishmania) mexicana', Cytokine, vol. 83, pp. 176-181. https://doi.org/10.1016/j.cyto.2016.04.016
Loria-Cervera, Elsy Nalleli ; Sosa-Bibiano, Erika Ivett ; Van Wynsberghe, Nicole Raymonde ; Saldarriaga, Omar Abdul ; Melby, Peter ; Andrade-Narvaez, Fernando Jose. / Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia : Cricetidae) infected by Leishmania (Leishmania) mexicana. In: Cytokine. 2016 ; Vol. 83. pp. 176-181.
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abstract = "Peromyscus yucatanicus, the main reservoir of Leishmania (Leishmania) mexicana in the Yucatan peninsula of Mexico, reproduces clinical and histological pictures of LCL in human as well as subclinical infection. Thus, we used this rodent as a novel experimental model. In this work, we analyzed cytokine mRNA expression in P. yucatanicus infected with L. (L.) mexicana. Animals were inoculated with either 2.5 × 106 or 1 × 102 promastigotes and cytokine expressions were analyzed by real-time RT-PCR in skin at 4 and 12 weeks post-infection (wpi). Independently of the parasite inoculum none of the infected rodents had clinical signs of LCL at 4 wpi and all expressed high IFN-γ mRNA. All P. yucatanicus inoculated with 2.5 × 106 promastigotes developed signs of LCL at 12 wpi while the mice inoculated with 1 × 102 remained subclinical. At that time, both IFN-γ and IL-10 were expressed in P. yucatanicus with clinical and subclinical infections. Expressions of TNF-α and IL-4 were significantly higher in clinical animals (2.5 × 106) compared with subclinical ones (1 × 102). High TGF-β expression was observed in P. yucatanicus with clinical signs when compared with healthy animals. Results suggested that the clinical course of L. (L.) mexicana infection in P. yucatanicus was associated with a specific local pattern of cytokine production at 12 wpi.",
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