Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity

Dai H. Chung, B. Mark Evers, Courtney Townsend, David Herndon, Tien C. Ko, Tatsuo Uchida, James C. Thompson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. The enzyme ornithine decarboxylase (ODC) catalyzes the rate-limiting step in polyamine biosynthesis and is important for gut mucosal repair after systemic injury (e.g., burns); however, the mechanisms responsible for the injury-mediated induction of ODC are not known. The purpose of this study was to determine whether interleukin-1 (IL-1) or tumor necrosis factor (TNF), which are released immediately after injury, regulates gut mucosal ODC enzyme activity and gene expression. Methods. In vivo: In experiment 1, 64 male BALB/c mice received either recombinant IL-1β (2 × 104 units/kg administered intraperitoneally) or saline solution. In experiment 2, 64 mice received either recombinant TNF-α (100 μg/kg administered intraperitoneally) or saline solution. We determined ODC enzyme activity and ODC mRNA levels in small intestine and kidneys at 2, 4, 12, and 24 hours after injection. In vitro: We also determined the ODC enzyme activity in intestinal epithelial crypt cells after either IL- 1β or TNF-α treatment. Results. IL-1, but not TNF, increased small intestinal ODC enzyme activity. In addition, IL-1 increased ODC enzyme activity in intestinal epithelial crypt cells at 5 and 6 hours after treatment. Both IL-1 and TNF increased small intestinal ODC mRNA levels. Neither agent affected ODC enzyme activity or ODC mRNA levels in the kidney. Conclusions. These results suggest that the cytokine-mediated induction of ODC in the small intestine is tissue specific, that the induction occurs at multiple cellular levels, and that ODC may play a vital role in the restoration of gut mucosa that occurs after injury.

Original languageEnglish (US)
Pages (from-to)364-369
Number of pages6
JournalSurgery
Volume112
Issue number2
StatePublished - 1992

Fingerprint

Ornithine Decarboxylase
Cytokines
Gene Expression
Enzymes
Interleukin-1
Tumor Necrosis Factor-alpha
Wounds and Injuries
Sodium Chloride
Messenger RNA
Small Intestine
Epithelial Cells
Kidney
Polyamines
Burns
Interleukin-2
Mucous Membrane

ASJC Scopus subject areas

  • Surgery

Cite this

Chung, D. H., Evers, B. M., Townsend, C., Herndon, D., Ko, T. C., Uchida, T., & Thompson, J. C. (1992). Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity. Surgery, 112(2), 364-369.

Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity. / Chung, Dai H.; Evers, B. Mark; Townsend, Courtney; Herndon, David; Ko, Tien C.; Uchida, Tatsuo; Thompson, James C.

In: Surgery, Vol. 112, No. 2, 1992, p. 364-369.

Research output: Contribution to journalArticle

Chung, DH, Evers, BM, Townsend, C, Herndon, D, Ko, TC, Uchida, T & Thompson, JC 1992, 'Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity', Surgery, vol. 112, no. 2, pp. 364-369.
Chung DH, Evers BM, Townsend C, Herndon D, Ko TC, Uchida T et al. Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity. Surgery. 1992;112(2):364-369.
Chung, Dai H. ; Evers, B. Mark ; Townsend, Courtney ; Herndon, David ; Ko, Tien C. ; Uchida, Tatsuo ; Thompson, James C. / Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity. In: Surgery. 1992 ; Vol. 112, No. 2. pp. 364-369.
@article{941f6d9dc2aa4cd781d6e1f50823ab80,
title = "Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity",
abstract = "Background. The enzyme ornithine decarboxylase (ODC) catalyzes the rate-limiting step in polyamine biosynthesis and is important for gut mucosal repair after systemic injury (e.g., burns); however, the mechanisms responsible for the injury-mediated induction of ODC are not known. The purpose of this study was to determine whether interleukin-1 (IL-1) or tumor necrosis factor (TNF), which are released immediately after injury, regulates gut mucosal ODC enzyme activity and gene expression. Methods. In vivo: In experiment 1, 64 male BALB/c mice received either recombinant IL-1β (2 × 104 units/kg administered intraperitoneally) or saline solution. In experiment 2, 64 mice received either recombinant TNF-α (100 μg/kg administered intraperitoneally) or saline solution. We determined ODC enzyme activity and ODC mRNA levels in small intestine and kidneys at 2, 4, 12, and 24 hours after injection. In vitro: We also determined the ODC enzyme activity in intestinal epithelial crypt cells after either IL- 1β or TNF-α treatment. Results. IL-1, but not TNF, increased small intestinal ODC enzyme activity. In addition, IL-1 increased ODC enzyme activity in intestinal epithelial crypt cells at 5 and 6 hours after treatment. Both IL-1 and TNF increased small intestinal ODC mRNA levels. Neither agent affected ODC enzyme activity or ODC mRNA levels in the kidney. Conclusions. These results suggest that the cytokine-mediated induction of ODC in the small intestine is tissue specific, that the induction occurs at multiple cellular levels, and that ODC may play a vital role in the restoration of gut mucosa that occurs after injury.",
author = "Chung, {Dai H.} and Evers, {B. Mark} and Courtney Townsend and David Herndon and Ko, {Tien C.} and Tatsuo Uchida and Thompson, {James C.}",
year = "1992",
language = "English (US)",
volume = "112",
pages = "364--369",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Cytokine regulation of gut ornithine decarboxylase gene expression and enzyme activity

AU - Chung, Dai H.

AU - Evers, B. Mark

AU - Townsend, Courtney

AU - Herndon, David

AU - Ko, Tien C.

AU - Uchida, Tatsuo

AU - Thompson, James C.

PY - 1992

Y1 - 1992

N2 - Background. The enzyme ornithine decarboxylase (ODC) catalyzes the rate-limiting step in polyamine biosynthesis and is important for gut mucosal repair after systemic injury (e.g., burns); however, the mechanisms responsible for the injury-mediated induction of ODC are not known. The purpose of this study was to determine whether interleukin-1 (IL-1) or tumor necrosis factor (TNF), which are released immediately after injury, regulates gut mucosal ODC enzyme activity and gene expression. Methods. In vivo: In experiment 1, 64 male BALB/c mice received either recombinant IL-1β (2 × 104 units/kg administered intraperitoneally) or saline solution. In experiment 2, 64 mice received either recombinant TNF-α (100 μg/kg administered intraperitoneally) or saline solution. We determined ODC enzyme activity and ODC mRNA levels in small intestine and kidneys at 2, 4, 12, and 24 hours after injection. In vitro: We also determined the ODC enzyme activity in intestinal epithelial crypt cells after either IL- 1β or TNF-α treatment. Results. IL-1, but not TNF, increased small intestinal ODC enzyme activity. In addition, IL-1 increased ODC enzyme activity in intestinal epithelial crypt cells at 5 and 6 hours after treatment. Both IL-1 and TNF increased small intestinal ODC mRNA levels. Neither agent affected ODC enzyme activity or ODC mRNA levels in the kidney. Conclusions. These results suggest that the cytokine-mediated induction of ODC in the small intestine is tissue specific, that the induction occurs at multiple cellular levels, and that ODC may play a vital role in the restoration of gut mucosa that occurs after injury.

AB - Background. The enzyme ornithine decarboxylase (ODC) catalyzes the rate-limiting step in polyamine biosynthesis and is important for gut mucosal repair after systemic injury (e.g., burns); however, the mechanisms responsible for the injury-mediated induction of ODC are not known. The purpose of this study was to determine whether interleukin-1 (IL-1) or tumor necrosis factor (TNF), which are released immediately after injury, regulates gut mucosal ODC enzyme activity and gene expression. Methods. In vivo: In experiment 1, 64 male BALB/c mice received either recombinant IL-1β (2 × 104 units/kg administered intraperitoneally) or saline solution. In experiment 2, 64 mice received either recombinant TNF-α (100 μg/kg administered intraperitoneally) or saline solution. We determined ODC enzyme activity and ODC mRNA levels in small intestine and kidneys at 2, 4, 12, and 24 hours after injection. In vitro: We also determined the ODC enzyme activity in intestinal epithelial crypt cells after either IL- 1β or TNF-α treatment. Results. IL-1, but not TNF, increased small intestinal ODC enzyme activity. In addition, IL-1 increased ODC enzyme activity in intestinal epithelial crypt cells at 5 and 6 hours after treatment. Both IL-1 and TNF increased small intestinal ODC mRNA levels. Neither agent affected ODC enzyme activity or ODC mRNA levels in the kidney. Conclusions. These results suggest that the cytokine-mediated induction of ODC in the small intestine is tissue specific, that the induction occurs at multiple cellular levels, and that ODC may play a vital role in the restoration of gut mucosa that occurs after injury.

UR - http://www.scopus.com/inward/record.url?scp=0026668631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026668631&partnerID=8YFLogxK

M3 - Article

VL - 112

SP - 364

EP - 369

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -