Cytokine secretion and latent herpes virus reactivation with 28 days of horizontal hypokinesia

Peter N. Uchakin, Raymond P. Stowe, Douglas Paddon-Jones, Brian W. Tobin, Arny A. Ferrando, Robert R. Wolfe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction: Hypokinesia is associated with spaceflight and prolonged illnesses and may lead to secondary immune deficiency. Methods: The distribution of immunocytes in whole blood, mitogen-induced cytokine secretion in vitro, Epstein-Barr virus (EBV) reactivation, and plasma Cortisol levels were studied in 13 healthy volunteers subjected to a horizontal bed rest (BR) regime for 28 d. Samples were collected before the study, weekly during BR, and then 3-5 d after the regime ended. Additionally, subjects were treated with hydrocortisone on the 1st and 27th d of BR to simulate the hypercortisolemia that occurs during stress. Results: The factors of 28-d BR regime accompanied by acute hypercortisolemia significantly decreased the relative and absolute number of total lymphocytes, CD3+ T-cells, T-helper subset, and monocytes, but increased the percentage of the CD8+ T-cells, and NK cells at the 4th wk compared with the baseline. A significant decrease in mitogen-activated secretion of IL-2, IFN-γ, TNF-β, IL-6, and IL-10 was registered at the same interval. Also, secretion of IL-2 and IFN-γ declined at the 2nd week of the BR regime. Secretion of IL-4 was significantly higher at the 2nd and 3rd weeks compared with the baseline. A significant increase in the shedding of EBV DNA in saliva was observed as early as the 3rd wk of BR. Conclusions: Stress factors associated with BR significantly alter immune responsiveness in vitro and in vivo. Changes in the cytokine secretion and cytokine imbalance precede latent EBV reactivation. PHA/LPS-activated cytokine secretion in whole blood can be used as a test system for predicting latent virus activation.

Original languageEnglish (US)
Pages (from-to)608-612
Number of pages5
JournalAviation Space and Environmental Medicine
Volume78
Issue number6
StatePublished - Jun 2007

Fingerprint

Hypokinesia
Bed Rest
Viruses
Cortisol
Cytokines
T-cells
Human Herpesvirus 4
Blood
Lymphocytes
Mitogens
Interleukin-2
Hydrocortisone
DNA
T-Lymphocytes
Chemical activation
Virus Activation
Space Flight
Plasmas
Lymphocyte Count
T-Lymphocyte Subsets

Keywords

  • Herpes virus
  • Immunity
  • Spaceflight
  • Stress

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Pollution
  • Medicine(all)

Cite this

Uchakin, P. N., Stowe, R. P., Paddon-Jones, D., Tobin, B. W., Ferrando, A. A., & Wolfe, R. R. (2007). Cytokine secretion and latent herpes virus reactivation with 28 days of horizontal hypokinesia. Aviation Space and Environmental Medicine, 78(6), 608-612.

Cytokine secretion and latent herpes virus reactivation with 28 days of horizontal hypokinesia. / Uchakin, Peter N.; Stowe, Raymond P.; Paddon-Jones, Douglas; Tobin, Brian W.; Ferrando, Arny A.; Wolfe, Robert R.

In: Aviation Space and Environmental Medicine, Vol. 78, No. 6, 06.2007, p. 608-612.

Research output: Contribution to journalArticle

Uchakin, Peter N. ; Stowe, Raymond P. ; Paddon-Jones, Douglas ; Tobin, Brian W. ; Ferrando, Arny A. ; Wolfe, Robert R. / Cytokine secretion and latent herpes virus reactivation with 28 days of horizontal hypokinesia. In: Aviation Space and Environmental Medicine. 2007 ; Vol. 78, No. 6. pp. 608-612.
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AU - Ferrando, Arny A.

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