Cytokine signature associated with disease severity in dengue

A. Raj Kumar Patro, Sriprasad Mohanty, Birendra K. Prusty, Diwakar K. Singh, Sagar Gaikwad, Tanuja Saswat, Soma Chattopadhyay, Bidyut K. Das, Rina Tripathy, Balachandran Ravindran

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dengue is the most rapidly spreading viral disease transmitted by the bite of infected Aedes mosquitos. The pathogenesis of dengue is still unclear; although host immune responses and virus serotypes have been proposed to contribute to disease severity. In this study, we examined the circulating dengue virus (DENV) and measured plasma levels of inflammatory mediators. Ninety-eight patients during a dengue outbreak in eastern India in 2016 were included in the study. The presence of DENV was demonstrated by detecting NS1 antigen; IgM capture ELISA and serotypes were discriminated by type-specific RT-PCR and/or sequencing. Plasma samples were assayed for 41-plex cytokine/chemokines using multiplex Luminex assay. Eighty-five (87%) samples were positive by NS1/IgM capture ELISA/RT-PCR. All four serotypes of DENV were detected in this outbreak, with DENV-2 as the predominant type, seen in 55% of cases. Mixed infections were seen in 39% of subjects. Among the host inflammatory biomarkers, GM-CSF, IFN-γ, IL-10, IL-15, IL-8, MCP-1, IL-6, MIP-1β, and TNF-α levels were significantly increased in dengue with and without warning signs, in severe dengue patients in comparison to healthy controls. Four cytokines IFN-γ, GM-CSF, IL-10, and MIP-1β correlated significantly with disease severity and could serve as potential predictor for disease severity. Information on the host biomarkers and the dengue serotype may help guide in optimizing effective intervention strategies.

Original languageEnglish (US)
Article number34
JournalViruses
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Dengue
Dengue Virus
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-10
Disease Outbreaks
Immunoglobulin M
Biomarkers
Enzyme-Linked Immunosorbent Assay
Severe Dengue
Interleukin-15
Polymerase Chain Reaction
Aedes
Virus Diseases
Bites and Stings
Culicidae
Coinfection
Interleukin-8
Chemokines
India

Keywords

  • Arbovirus
  • Cytokine
  • Dengue
  • Inflammation
  • Luminex-bead assay
  • Severe dengue

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Raj Kumar Patro, A., Mohanty, S., Prusty, B. K., Singh, D. K., Gaikwad, S., Saswat, T., ... Ravindran, B. (2019). Cytokine signature associated with disease severity in dengue. Viruses, 11(1), [34]. https://doi.org/10.3390/v11010034

Cytokine signature associated with disease severity in dengue. / Raj Kumar Patro, A.; Mohanty, Sriprasad; Prusty, Birendra K.; Singh, Diwakar K.; Gaikwad, Sagar; Saswat, Tanuja; Chattopadhyay, Soma; Das, Bidyut K.; Tripathy, Rina; Ravindran, Balachandran.

In: Viruses, Vol. 11, No. 1, 34, 01.01.2019.

Research output: Contribution to journalArticle

Raj Kumar Patro, A, Mohanty, S, Prusty, BK, Singh, DK, Gaikwad, S, Saswat, T, Chattopadhyay, S, Das, BK, Tripathy, R & Ravindran, B 2019, 'Cytokine signature associated with disease severity in dengue', Viruses, vol. 11, no. 1, 34. https://doi.org/10.3390/v11010034
Raj Kumar Patro A, Mohanty S, Prusty BK, Singh DK, Gaikwad S, Saswat T et al. Cytokine signature associated with disease severity in dengue. Viruses. 2019 Jan 1;11(1). 34. https://doi.org/10.3390/v11010034
Raj Kumar Patro, A. ; Mohanty, Sriprasad ; Prusty, Birendra K. ; Singh, Diwakar K. ; Gaikwad, Sagar ; Saswat, Tanuja ; Chattopadhyay, Soma ; Das, Bidyut K. ; Tripathy, Rina ; Ravindran, Balachandran. / Cytokine signature associated with disease severity in dengue. In: Viruses. 2019 ; Vol. 11, No. 1.
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