Cytomorphology and B72.3 labeling of benign and malignant ductal epithelium in pancreatic lesions compared to gastrointestinal epithelium

Ranjana Nawgiri, Julie A. Nagle, David C. Wilbur, Martha B. Pitman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Endoscopic ultrasound-guided pancreatic fine-needle aspiration biopsy very frequently produces gastrointestinal epithelial contamination (GIC). We studied the cytomorphology and B72.3 immunoreactivity of lesional epithelium of benign and malignant ductal lesions of the pancreas and compared the findings to our previously established template of GIC. Air-dried smears, fixed smears, and ThinPrep® (TP) specimens were obtained using a cytobrush, directly from benign and malignant ductal lesions of 18 Whipple specimens, to ensure purity of the epithelium studied. Smear background, cell architecture, and cellular features were analyzed. Immunocytochemical staining with B72.3 was performed in 14 cases. Epithelium of ductal carcinoma was distinguished from benign ductal epithelium in chronic pancreatitis and GIC primarily by crowded architecture and atypical cellular features, including high nuclear-to-cytoplasmic ratio, irregular nuclei, nucleoli, and vacuolated cytoplasm. Benign ductal and GIC epithelium were only distinguished by architecture (goblet cells and brush borders), but not consistently, especially gastric epithelium that lacked these features. B72.3 shows promise in the differentiation between GIC and benign and malignant ductal epithelium, with no staining supporting benign ductal cells, fine punctate perinuclear staining correlating with GIC, and strong cytoplasmic staining supporting malignancy.

Original languageEnglish (US)
Pages (from-to)300-305
Number of pages6
JournalDiagnostic Cytopathology
Volume35
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Epithelium
Staining and Labeling
Endoscopic Ultrasound-Guided Fine Needle Aspiration
Ductal Carcinoma
Goblet Cells
Chronic Pancreatitis
Microvilli
Fine Needle Biopsy
B72.3 antibody
Pancreas
Stomach
Cytoplasm
Air
Neoplasms

Keywords

  • B72.3
  • Endoscopic ultrasound-guided fine-needle aspiration biopsy
  • Gastrointestinal contamination
  • Pancreas
  • Pancreatic cancer

ASJC Scopus subject areas

  • Anatomy

Cite this

Cytomorphology and B72.3 labeling of benign and malignant ductal epithelium in pancreatic lesions compared to gastrointestinal epithelium. / Nawgiri, Ranjana; Nagle, Julie A.; Wilbur, David C.; Pitman, Martha B.

In: Diagnostic Cytopathology, Vol. 35, No. 5, 05.2007, p. 300-305.

Research output: Contribution to journalArticle

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