Cytoplasmic dynein is a minus end-directed motor for membranous organelles

Trina A. Schroer, Eric R. Steuer, Michael Sheetz

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Abstract

The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a high-speed cytosol caused the movement of purified organelles on microtubules predominantly in the minus end direction. Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90%. Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement. We propose that cytoplasmic dynein is the motor for minus end-directed organelle movement, but that additional cytosolic factors are also required to produce organelle motility.

Original languageEnglish (US)
Pages (from-to)937-946
Number of pages10
JournalCell
Volume56
Issue number6
DOIs
StatePublished - Mar 24 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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