Cytoplasmic dynein is a minus end-directed motor for membranous organelles

Trina A. Schroer, Eric R. Steuer, Michael Sheetz

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a high-speed cytosol caused the movement of purified organelles on microtubules predominantly in the minus end direction. Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90%. Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement. We propose that cytoplasmic dynein is the motor for minus end-directed organelle movement, but that additional cytosolic factors are also required to produce organelle motility.

Original languageEnglish (US)
Pages (from-to)937-946
Number of pages10
JournalCell
Volume56
Issue number6
DOIs
StatePublished - Mar 24 1989
Externally publishedYes

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Cytoplasmic Dyneins
Organelles
Cytosol
Microtubules
Vanadates
Fibroblasts
Assays
Chick Embryo

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Cytoplasmic dynein is a minus end-directed motor for membranous organelles. / Schroer, Trina A.; Steuer, Eric R.; Sheetz, Michael.

In: Cell, Vol. 56, No. 6, 24.03.1989, p. 937-946.

Research output: Contribution to journalArticle

Schroer, Trina A. ; Steuer, Eric R. ; Sheetz, Michael. / Cytoplasmic dynein is a minus end-directed motor for membranous organelles. In: Cell. 1989 ; Vol. 56, No. 6. pp. 937-946.
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