Cytoprotection of Human Dermal Fibroblasts against Silver Sulfadiazine Using Recombinant Growth Factors

Robert L. McCauley, Ying Yue Li, Vimlariani Chopra, David Herndon, Martin C. Robson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Topical antimicrobial agents, silver sulfadiazine (SSD) and mafenide acetate (MA), have been associated with delayed wound healing. Previous in vitro studies with human dermal fibroblasts (HDF) have shown progressive cellular cytotoxicity with increasing concentrations of SSD and MA. However, preexposure of HDF to epidermal growth factor, basic fibroblast growth factor, or platelet-derived growth factor has resulted in cytoprotection of HDF against 0.01 and 0.03% concentrations of SSD as determined by phase-contrast microscopy (PCM), hemocytometer cell counts, and total cellular protein content. PCM, however, showed slower destruction of HDF at the 0.05% concentration of SSD. These data suggest that cells activated by growth factors either take up less SSD or are more resistant to the direct cytotoxic effects of this drug.

Original languageEnglish (US)
Pages (from-to)378-384
Number of pages7
JournalJournal of Surgical Research
Volume56
Issue number4
DOIs
StatePublished - Apr 1994

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Silver Sulfadiazine
Cytoprotection
Intercellular Signaling Peptides and Proteins
Fibroblasts
Mafenide
Skin
Phase-Contrast Microscopy
Platelet-Derived Growth Factor
Fibroblast Growth Factor 2
Anti-Infective Agents
Epidermal Growth Factor
Wound Healing
Cell Count
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Cytoprotection of Human Dermal Fibroblasts against Silver Sulfadiazine Using Recombinant Growth Factors. / McCauley, Robert L.; Li, Ying Yue; Chopra, Vimlariani; Herndon, David; Robson, Martin C.

In: Journal of Surgical Research, Vol. 56, No. 4, 04.1994, p. 378-384.

Research output: Contribution to journalArticle

McCauley, Robert L. ; Li, Ying Yue ; Chopra, Vimlariani ; Herndon, David ; Robson, Martin C. / Cytoprotection of Human Dermal Fibroblasts against Silver Sulfadiazine Using Recombinant Growth Factors. In: Journal of Surgical Research. 1994 ; Vol. 56, No. 4. pp. 378-384.
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