TY - JOUR
T1 - Cytoprotection of human dermal fibroblasts against silver sulfadiazine using recombinant growth factors
AU - McCauley, Robert L.
AU - Li, Ying Yue
AU - Chopra, Vimlariani
AU - Herndon, David N.
AU - Robson, Martin C.
PY - 1994/4
Y1 - 1994/4
N2 - Topical antimicrobial agents, silver sulfadiazine (SSD) and mafenide acetate (MA), have been associated with delayed wound healing. Previous in vitro studies with human dermal fibroblasts (HDF) have shown progressive cellular cytotoxicity with increasing concentrations of SSD and MA. However, preexposure of HDF to epidermal growth factor, basic fibroblast growth factor, or platelet-derived growth factor has resulted in cytoprotection of HDF against 0.01 and 0.03% concentrations of SSD as determined by phase-contrast microscopy (PCM), hemocytometer cell counts, and total cellular protein content. PCM, however, showed slower destruction of HDF at the 0.05% concentration of SSD. These data suggest that cells activated by growth factors either take up less SSD or are more resistant to the direct cytotoxic effects of this drug.
AB - Topical antimicrobial agents, silver sulfadiazine (SSD) and mafenide acetate (MA), have been associated with delayed wound healing. Previous in vitro studies with human dermal fibroblasts (HDF) have shown progressive cellular cytotoxicity with increasing concentrations of SSD and MA. However, preexposure of HDF to epidermal growth factor, basic fibroblast growth factor, or platelet-derived growth factor has resulted in cytoprotection of HDF against 0.01 and 0.03% concentrations of SSD as determined by phase-contrast microscopy (PCM), hemocytometer cell counts, and total cellular protein content. PCM, however, showed slower destruction of HDF at the 0.05% concentration of SSD. These data suggest that cells activated by growth factors either take up less SSD or are more resistant to the direct cytotoxic effects of this drug.
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U2 - 10.1006/jsre.1994.1059
DO - 10.1006/jsre.1994.1059
M3 - Article
C2 - 8152234
AN - SCOPUS:0028223354
SN - 0022-4804
VL - 56
SP - 378
EP - 384
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 4
ER -