Cytosolic Gαs acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth

Jiang Zhou Yu, Rahul H. Dave, John Allen, Tulika Sarma, Mark M. Rasenick

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

It is now evident that Gαs traffics into cytosol following G protein-coupled receptor activation, and α subunits of some heterotrimeric G-proteins, including Gαs bind to tubulin in vitro. Nevertheless, many features of G-protein-microtubule interaction and possible intracellular effects of G protein α subunits remain unclear. In this study, several biochemical approaches demonstrated that activated Gαs directly bound to tubulin and cellular microtubules, and fluorescence microscopy showed that cholera toxin-activated Gαs colocalized with microtubules. The activated, GTP-bound, Gαs mimicked tubulin in serving as a GTPase activator for β-tubulin. As a result, activated Gαs made microtubules more dynamic, both in vitro and in cells, decreasing the pool of insoluble microtubules without changing total cellular tubulin content. The amount of acetylated tubulin (an indicator of microtubule stability) was reduced in the presence of Gαs activated by mutation. Previous studies showed that cholera toxin and cAMP analogs may stimulate neurite outgrowth in PC12 cells. However, in this study, overexpression of a constitutively activated Gαs or activation of Gαs with cholera toxin in protein kinase A-deficient PC12 cells promoted neurite outgrowth in a cAMP-independent manner. Thus, it is suggested that activated Gαs acts as an intracellular messenger to regulate directly microtubule dynamics and promote neurite outgrowth. These data serve to link G-protein signaling with modulation of the cytoskeleton and cell morphology.

Original languageEnglish (US)
Pages (from-to)10462-10472
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number16
DOIs
StatePublished - Apr 17 2009
Externally publishedYes

Fingerprint

Tubulin
Microtubules
Cholera Toxin
GTP-Binding Proteins
PC12 Cells
GTP Phosphohydrolase Activators
Chemical activation
Heterotrimeric GTP-Binding Proteins
Fluorescence microscopy
Protein Subunits
G-Protein-Coupled Receptors
Cyclic AMP-Dependent Protein Kinases
Guanosine Triphosphate
Telecommunication links
Neuronal Outgrowth
Cytoskeleton
Fluorescence Microscopy
Cytosol
Modulation
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Cytosolic Gαs acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth. / Yu, Jiang Zhou; Dave, Rahul H.; Allen, John; Sarma, Tulika; Rasenick, Mark M.

In: Journal of Biological Chemistry, Vol. 284, No. 16, 17.04.2009, p. 10462-10472.

Research output: Contribution to journalArticle

Yu, Jiang Zhou ; Dave, Rahul H. ; Allen, John ; Sarma, Tulika ; Rasenick, Mark M. / Cytosolic Gαs acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 16. pp. 10462-10472.
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