De novo design and spectroscopic characterization of a dinucleating copper-binding pentadecapeptide

David A. Rockcliffe, Arthur Cammers, Ayaluru Murali, William Russell, Victoria J. DeRose

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A spectroscopic study of aqueous solutions of Ac-WGHGHGHGPGHGHGH-NH 2 (HGP) indicates that copper(II) binds to the peptide to form a 2:1 Cu2+/HGP complex with four nitrogen atoms in the copper coordination environment. Electron paramagnetic resonance (EPR) and UV-visible data suggest copper binding through the peptide backbone and imidazole nitrogen donors. Circular dichroism data show that HGP is unbound below pH 5.5 and is copper-saturated at pH 9 and above. The apo form of the peptide is unstructured in solution and is organized into a turn conformation in the presence of 2 mol equiv of Cu2+ at basic pH. EPR measurements for 2:1 Cu 2+/HGP solutions in the g = 2 region and within the pH range 7-11 exhibit axial spectra. A molecular-mechanics-minimized model of the Cu 2+/HGP complex gave a Cu⋯Cu separation of 8 Å.

Original languageEnglish (US)
Pages (from-to)472-474
Number of pages3
JournalInorganic Chemistry
Volume45
Issue number2
DOIs
StatePublished - Jan 23 2006
Externally publishedYes

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Copper
peptides
copper
Peptides
Paramagnetic resonance
electron paramagnetic resonance
Nitrogen
Molecular mechanics
imidazoles
nitrogen atoms
dichroism
Conformations
aqueous solutions
nitrogen
Atoms

ASJC Scopus subject areas

  • Inorganic Chemistry

Cite this

De novo design and spectroscopic characterization of a dinucleating copper-binding pentadecapeptide. / Rockcliffe, David A.; Cammers, Arthur; Murali, Ayaluru; Russell, William; DeRose, Victoria J.

In: Inorganic Chemistry, Vol. 45, No. 2, 23.01.2006, p. 472-474.

Research output: Contribution to journalArticle

Rockcliffe, David A. ; Cammers, Arthur ; Murali, Ayaluru ; Russell, William ; DeRose, Victoria J. / De novo design and spectroscopic characterization of a dinucleating copper-binding pentadecapeptide. In: Inorganic Chemistry. 2006 ; Vol. 45, No. 2. pp. 472-474.
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