Deafness due to degeneration of cochlear neurons in caspase-3-deficient mice

Hirofumi Morishita, Tomoko Makishima, Chie Kaneko, Yun Shain Lee, Neil Segil, Katsuhiko Takahashi, Akio Kuraoka, Takashi Nakagawa, Junichi Nabekura, Keiko Nakayama, Kei Ichi Nakayama

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Mice that lack caspase-3, which functions in apoptosis, were generated by gene targeting and shown to undergo hearing loss. The ABR threshold of the caspase-3-/- mice was significantly elevated compared to that of caspase-3+/+ mice at 15 days of age and was progressively elevated further by 30 days. Distortion product otoacoustic emissions were not detectable in caspase-3-/- mice at 15 days of age. Caspase-3-/- mice exhibited marked degeneration of spiral ganglion neurons and a loss of inner and outer hair cells in the cochlea at 30 days of age, although no such changes were apparent at 15 days. The degenerating neurons manifested features, including cytoplasmic vacuolization, distinct from those characteristic of apoptosis. Spiral ganglion neurons and cochlear hair cells thus appear to require caspase-3 for survival but not for initial development. The mapping of both the human caspase-3 gene and the locus responsible for an autosomal dominant, nonsyndromic form of hearing loss (DFNA24) to chromosome 4q35 suggests that the caspase-3-/- mice may represent a model of this human condition,

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number1
DOIs
StatePublished - 2001

Keywords

  • ABR
  • Apoptosis
  • Caspase-3
  • DFNA24
  • DPOAE
  • Gene targeting
  • Hair cell
  • Hearing loss
  • Spiral ganglion
  • Vacuolization

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Morishita, H., Makishima, T., Kaneko, C., Lee, Y. S., Segil, N., Takahashi, K., Kuraoka, A., Nakagawa, T., Nabekura, J., Nakayama, K., & Nakayama, K. I. (2001). Deafness due to degeneration of cochlear neurons in caspase-3-deficient mice. Biochemical and Biophysical Research Communications, 284(1), 142-149. https://doi.org/10.1006/bbrc.2001.4939