Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform

Nittaya Khakhum, Noe Baruch-Torres, Jacob L. Stockton, Itziar Chapartegui Gonzalez, Alexander J. Badten, Awadalkareem Adam, Tian Wang, Alejandro Huerta-Saquero, Y. Whitney Yin, Alfredo G. Torres

Research output: Contribution to journalArticlepeer-review

Abstract

Virus-like particles (VLPs) are protein-based nanoparticles frequently used as carriers in conjugate vaccine platforms. VLPs have been used to display foreign antigens for vaccination and to deliver immunotherapy against diseases. Hemolysin-coregulated proteins 1 (Hcp1) is a protein component of the Burkholderia type 6 secretion system, which participates in intracellular invasion and dissemination. This protein has been reported as a protective antigen and is used in multiple vaccine candidates with various platforms against melioidosis, a severe infectious disease caused by the intracellular pathogen Burkholderia pseudomallei. In this study, we used P22 VLPs as a surface platform for decoration with Hcp1 using chemical conjugation. C57BL/6 mice were intranasally immunized with three doses of either PBS, VLPs, or conjugated Hcp1-VLPs. Immunization with Hcp1-VLPs formulation induced Hcp1-specific IgG, IgG1, IgG2c, and IgA antibody responses. Furthermore, the serum from Hcp1-VLPs immunized mice enhanced the bacterial uptake and opsonophagocytosis by macrophages in the presence of complement. This study demonstrated an alternative strategy to develop a VLPs-based vaccine platform against Burkholderia species.

Original languageEnglish (US)
JournalInfection and immunity
Volume92
Issue number3
DOIs
StatePublished - Mar 2024
Externally publishedYes

Keywords

  • B. pseudomallei
  • Hcp1
  • melioidosis
  • P22-based nanovaccines
  • type 6 secretion system
  • virus-like particles

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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