Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat

Patrick M. Callahan, Joseph M. Paris, Kathryn Cunningham, Patricia Shinnick-Gallagher

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls. fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.

Original languageEnglish (US)
Pages (from-to)335-339
Number of pages5
JournalBrain Research
Volume555
Issue number2
DOIs
StatePublished - Aug 2 1991

Fingerprint

GABAergic Neurons
Amygdala
gamma-Aminobutyric Acid
Pyramidal Cells
Sprague Dawley Rats
Electrodes
Seizures
Neurons
Brain

Keywords

  • Amygdala
  • Electrical kindling
  • Epilepsy
  • Immunohistochemistry
  • Rat
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat. / Callahan, Patrick M.; Paris, Joseph M.; Cunningham, Kathryn; Shinnick-Gallagher, Patricia.

In: Brain Research, Vol. 555, No. 2, 02.08.1991, p. 335-339.

Research output: Contribution to journalArticle

Callahan, Patrick M. ; Paris, Joseph M. ; Cunningham, Kathryn ; Shinnick-Gallagher, Patricia. / Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat. In: Brain Research. 1991 ; Vol. 555, No. 2. pp. 335-339.
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